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- W2623017803 abstract "Abstract There does not currently exist a standardized indicator of how well a cryo-EM-derived model represents the density from which it was generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected model–model agreement criterion for EM maps reported to be at 5 Å resolution or better. Associating multiple atomic models with a deposited EM map provides a rapid and accessible reporter of convergence, a strong indicator of highly resolved molecular detail, and is an important step toward an FSC-independent assessment of map and model quality." @default.
- W2623017803 created "2017-06-15" @default.
- W2623017803 creator A5023367359 @default.
- W2623017803 creator A5051355898 @default.
- W2623017803 creator A5063672303 @default.
- W2623017803 date "2017-04-19" @default.
- W2623017803 modified "2023-10-14" @default.
- W2623017803 title "A multi-model approach to assessing local and global cryo-EM map quality" @default.
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- W2623017803 doi "https://doi.org/10.1101/128561" @default.
- W2623017803 hasPublicationYear "2017" @default.
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