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• W2623473458 startingPage "833" @default.
• W2623473458 abstract "Agave fructans are increasingly important in food industry and nutrition sciences as a potential ingredient of functional food, thus practical analysis tools to characterize them are needed. In view of the importance of the molecular weight on the functional properties of agave fructans, this study has the purpose to optimize a method to determine their molecular weight distribution by HPLC-SEC for industrial application. The optimization was carried out using a simplex method. The optimum conditions obtained were at column temperature of 61.7 °C using tri-distilled water without salt, adjusted pH of 5.4 and a flow rate of 0.36 mL/min. The exclusion range is from 1 to 49 of polymerization degree (180–7966 Da). This proposed method represents an accurate and fast alternative to standard methods involving multiple-detection or hydrolysis of fructans. The industrial applications of this technique might be for quality control, study of fractionation processes and determination of purity." @default.
• W2623473458 created "2017-06-15" @default.
• W2623473458 creator A5043831173 @default.
• W2623473458 creator A5053709747 @default.
• W2623473458 creator A5054997352 @default.
• W2623473458 creator A5059210322 @default.
• W2623473458 date "2017-12-01" @default.
• W2623473458 modified "2023-10-18" @default.
• W2623473458 title "Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans" @default.
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• W2623473458 doi "https://doi.org/10.1016/j.foodchem.2017.06.020" @default.
• W2623473458 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28764075" @default.
• W2623473458 hasPublicationYear "2017" @default.
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