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• W2623910725 abstract "Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/dCas9 system has been widely applied in both transcriptional regulation and epigenetic studies. However, for multiple targets, independent expression of multiple single guide RNAs (sgRNAs) is needed, which is less convenient. To address the problem, we employed a DNase-dead Cpf1 mutant (ddCpf1) for multiplex gene regulation. We demonstrated that ddCpf1 alone could be employed for gene repression in Escherichia coli , and the repression was more effective with CRISPR RNAs (crRNAs) specifically targeting to the template strand of its target genes, which was different from that of dCas9. When targeting the promoter region, both strands showed effective repression by the ddCpf1/crRNA complex. The whole-transcriptome RNA-seq technique was further employed to demonstrate the high specificity of ddCpf1-mediated repression. Besides, we proved that the remaining RNase activity in ddCpf1 was capable of processing a precursor CRISPR array to simply generate multiple mature crRNAs in vivo , facilitating multiplex gene regulation. With the employment of this multiplex gene regulation strategy, we also showed how to quickly screen a library of candidate targets, that is, the two-component systems in E. coli . Therefore, based on our findings here, the CRISPR-ddCpf1 system may be further developed and widely applied in both biological research and clinical studies." @default.
• W2623910725 created "2017-06-15" @default.
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• W2623910725 date "2017-06-06" @default.
• W2623910725 modified "2023-10-16" @default.
• W2623910725 title "Multiplex gene regulation by CRISPR-ddCpf1" @default.
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• W2623910725 doi "https://doi.org/10.1038/celldisc.2017.18" @default.
• W2623910725 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5460296" @default.
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• W2623910725 hasPublicationYear "2017" @default.
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