Matches in SemOpenAlex for { <https://semopenalex.org/work/W2624125560> ?p ?o ?g. }
- W2624125560 endingPage "491" @default.
- W2624125560 startingPage "479" @default.
- W2624125560 abstract "Preventing the spread of infectious diseases remains an urgent priority worldwide, and this is driving the development of advanced nanotechnology to diagnose infections at the point of care. Herein, we report the creation of a library of novel nanobody capture ligands to detect p24, one of the earliest markers of HIV infection. We demonstrate that these nanobodies, one tenth the size of conventional antibodies, exhibit high sensitivity and broad specificity to global HIV-1 subtypes. Biophysical characterization indicates strong 690 pM binding constants and fast kinetic on-rates, 1 to 2 orders of magnitude better than monoclonal antibody comparators. A crystal structure of the lead nanobody and p24 was obtained and used alongside molecular dynamics simulations to elucidate the molecular basis of these enhanced performance characteristics. They indicate that binding occurs at C-terminal helices 10 and 11 of p24, a negatively charged region of p24 complemented by the positive surface of the nanobody binding interface involving CDR1, CDR2, and CDR3 loops. Our findings have broad implications on the design of novel antibodies and a wide range of advanced biomedical applications." @default.
- W2624125560 created "2017-06-15" @default.
- W2624125560 creator A5020153149 @default.
- W2624125560 creator A5021532425 @default.
- W2624125560 creator A5027445812 @default.
- W2624125560 creator A5035620868 @default.
- W2624125560 creator A5046183614 @default.
- W2624125560 creator A5047563153 @default.
- W2624125560 creator A5053060809 @default.
- W2624125560 creator A5068528573 @default.
- W2624125560 creator A5071280884 @default.
- W2624125560 creator A5072171174 @default.
- W2624125560 creator A5072374047 @default.
- W2624125560 creator A5076683134 @default.
- W2624125560 creator A5081104745 @default.
- W2624125560 creator A5086202396 @default.
- W2624125560 date "2017-06-07" @default.
- W2624125560 modified "2023-10-16" @default.
- W2624125560 title "Unravelling the Molecular Basis of High Affinity Nanobodies against HIV p24: <i>In Vitro</i> Functional, Structural, and <i>in Silico</i> Insights" @default.
- W2624125560 cites W1487420490 @default.
- W2624125560 cites W1592986776 @default.
- W2624125560 cites W1953822958 @default.
- W2624125560 cites W1976784576 @default.
- W2624125560 cites W1977038143 @default.
- W2624125560 cites W1979013615 @default.
- W2624125560 cites W1979780600 @default.
- W2624125560 cites W1980758834 @default.
- W2624125560 cites W1985534966 @default.
- W2624125560 cites W1985731056 @default.
- W2624125560 cites W1986764354 @default.
- W2624125560 cites W1994287518 @default.
- W2624125560 cites W1998292458 @default.
- W2624125560 cites W2008462557 @default.
- W2624125560 cites W2015237638 @default.
- W2624125560 cites W2028400386 @default.
- W2624125560 cites W2029667189 @default.
- W2624125560 cites W2031989831 @default.
- W2624125560 cites W2033079214 @default.
- W2624125560 cites W2037042031 @default.
- W2624125560 cites W2038840577 @default.
- W2624125560 cites W2040004074 @default.
- W2624125560 cites W2042649563 @default.
- W2624125560 cites W2043219011 @default.
- W2624125560 cites W2044172327 @default.
- W2624125560 cites W2051096704 @default.
- W2624125560 cites W2057278101 @default.
- W2624125560 cites W2076965602 @default.
- W2624125560 cites W2078109757 @default.
- W2624125560 cites W2079655614 @default.
- W2624125560 cites W2089788910 @default.
- W2624125560 cites W2095553811 @default.
- W2624125560 cites W2097202353 @default.
- W2624125560 cites W2100355920 @default.
- W2624125560 cites W2104178116 @default.
- W2624125560 cites W2106854762 @default.
- W2624125560 cites W2113320844 @default.
- W2624125560 cites W2124026197 @default.
- W2624125560 cites W2125771887 @default.
- W2624125560 cites W2127917585 @default.
- W2624125560 cites W2130675979 @default.
- W2624125560 cites W2132629607 @default.
- W2624125560 cites W2138713450 @default.
- W2624125560 cites W2142642621 @default.
- W2624125560 cites W2144413761 @default.
- W2624125560 cites W2150135863 @default.
- W2624125560 cites W2150981663 @default.
- W2624125560 cites W2152345838 @default.
- W2624125560 cites W2152505869 @default.
- W2624125560 cites W2159725619 @default.
- W2624125560 cites W2160157393 @default.
- W2624125560 cites W2160544821 @default.
- W2624125560 cites W2161529001 @default.
- W2624125560 cites W2163341755 @default.
- W2624125560 cites W2165148980 @default.
- W2624125560 cites W2180229411 @default.
- W2624125560 cites W2412092132 @default.
- W2624125560 cites W2470140135 @default.
- W2624125560 cites W2517950264 @default.
- W2624125560 cites W2535014449 @default.
- W2624125560 cites W2578150941 @default.
- W2624125560 cites W4248872320 @default.
- W2624125560 doi "https://doi.org/10.1021/acsinfecdis.6b00189" @default.
- W2624125560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28591513" @default.
- W2624125560 hasPublicationYear "2017" @default.
- W2624125560 type Work @default.
- W2624125560 sameAs 2624125560 @default.
- W2624125560 citedByCount "33" @default.
- W2624125560 countsByYear W26241255602017 @default.
- W2624125560 countsByYear W26241255602018 @default.
- W2624125560 countsByYear W26241255602019 @default.
- W2624125560 countsByYear W26241255602020 @default.
- W2624125560 countsByYear W26241255602021 @default.
- W2624125560 countsByYear W26241255602022 @default.
- W2624125560 countsByYear W26241255602023 @default.
- W2624125560 crossrefType "journal-article" @default.
- W2624125560 hasAuthorship W2624125560A5020153149 @default.
- W2624125560 hasAuthorship W2624125560A5021532425 @default.
- W2624125560 hasAuthorship W2624125560A5027445812 @default.