FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2624729432> ?p ?o ?g. }

• W2624729432 endingPage "584" @default.
• W2624729432 startingPage "584" @default.
• W2624729432 abstract "Studies indicate that high-fat diets can have adverse effects on ovarian activity and that aging can reduce female fertility due to decreased quality and size of the oocyte ovarian reserve. The PI3K/AKT1 signaling pathway and the FOXO3a transcription factor are important regulators of cell proliferation and survival and are involved in the activation of primordial follicles. The aim of the current study was to determine the effects of a high-fat and energy diet on ovarian gene expression (AMH, IGF-1, AKT1, PI3K, MTOR, BMP15, CAT, SIRT1 e FOXO3) in young and aged female mice. Twenty female mice (C57BL/6) at four and thirteen months of age were used (young: n = 10 and old: n = 10). Animals were divided into four groups: young/control diet (YC); young/high fat diet (YH); old/control diet (OC) and old/high-fat diet (OH). Both diets contained 14.1% crude protein, but the control diet had 75.9% carbohydrate, 10.0% fat and 3,061 kcal/100g of energy, while the high-fat diet contained 54.6%; 32.7% and 4,402/100g kcal, respectively. Females were fed the diets during 55 days, intake was measured every two days and body weight measured weekly. After euthanasia, ovaries were collected, RNA was extracted using the Trizol method (Trizol, Invitrogen, USA), RNA was converted to cDNA (Biorad, , Hercules, CA, EUA) and the expression of target genes measured by qRT-PCR (Applied Biosystems, Foster City, CA, USA) using the β2 - microgobulina as the endogenous control. For statistical analysis data were compared by two-way ANOVA (GraphPad Software Inc., La Jolla, CA, USA) for testing the effect of age, diet and its interaction. AMH was 67% less expressed in old than in young females (P<0.05). Furthermore, there was a 46% reduction in the expression of IGF-1 in females fed the high-fat diet (P<0.05), as well as lower food intake when compared to mice fed the control diet (P<0.05; 2.9 ± 0.1 and 3.9 ± 0.1). Previous studies suggest that IGF-1 may be involved in the premature depletion of the ovarian reserve (Schneider et al., 2015; J Ovarian Res. 7: 120). Females fed the high-fat diet had increased body weight gain compared to controls (P<0.05; 28% and 9% gain, respectively). In conclusion, there was a reduction in IGF-1 expression in the ovary of females fed the high fat diet, which may be a factor modulating ovarian aging." @default.
• W2624729432 created "2017-06-23" @default.
• W2624729432 creator A5018668012 @default.
• W2624729432 creator A5021008021 @default.
• W2624729432 creator A5038603332 @default.
• W2624729432 creator A5050502418 @default.
• W2624729432 creator A5052146593 @default.
• W2624729432 creator A5056321390 @default.
• W2624729432 creator A5057277899 @default.
• W2624729432 creator A5073868984 @default.
• W2624729432 date "2015-03-01" @default.
• W2624729432 modified "2023-09-27" @default.
• W2624729432 title "Effects of a high-fat and energy diet on ovarian gene expression in young and aged female mice" @default.
• W2624729432 hasPublicationYear "2015" @default.
• W2624729432 type Work @default.
• W2624729432 sameAs 2624729432 @default.
• W2624729432 citedByCount "0" @default.
• W2624729432 crossrefType "journal-article" @default.
• W2624729432 hasAuthorship W2624729432A5018668012 @default.
• W2624729432 hasAuthorship W2624729432A5021008021 @default.
• W2624729432 hasAuthorship W2624729432A5038603332 @default.
• W2624729432 hasAuthorship W2624729432A5050502418 @default.
• W2624729432 hasAuthorship W2624729432A5052146593 @default.
• W2624729432 hasAuthorship W2624729432A5056321390 @default.
• W2624729432 hasAuthorship W2624729432A5057277899 @default.
• W2624729432 hasAuthorship W2624729432A5073868984 @default.
• W2624729432 hasConcept C104317684 @default.
• W2624729432 hasConcept C112190416 @default.
• W2624729432 hasConcept C126322002 @default.
• W2624729432 hasConcept C134018914 @default.
• W2624729432 hasConcept C150194340 @default.
• W2624729432 hasConcept C16685009 @default.
• W2624729432 hasConcept C168003608 @default.
• W2624729432 hasConcept C205713591 @default.
• W2624729432 hasConcept C2777203538 @default.
• W2624729432 hasConcept C2778324911 @default.
• W2624729432 hasConcept C55493867 @default.
• W2624729432 hasConcept C71924100 @default.
• W2624729432 hasConcept C86339819 @default.
• W2624729432 hasConcept C86803240 @default.
• W2624729432 hasConceptScore W2624729432C104317684 @default.
• W2624729432 hasConceptScore W2624729432C112190416 @default.
• W2624729432 hasConceptScore W2624729432C126322002 @default.
• W2624729432 hasConceptScore W2624729432C134018914 @default.
• W2624729432 hasConceptScore W2624729432C150194340 @default.
• W2624729432 hasConceptScore W2624729432C16685009 @default.
• W2624729432 hasConceptScore W2624729432C168003608 @default.
• W2624729432 hasConceptScore W2624729432C205713591 @default.
• W2624729432 hasConceptScore W2624729432C2777203538 @default.
• W2624729432 hasConceptScore W2624729432C2778324911 @default.
• W2624729432 hasConceptScore W2624729432C55493867 @default.
• W2624729432 hasConceptScore W2624729432C71924100 @default.
• W2624729432 hasConceptScore W2624729432C86339819 @default.
• W2624729432 hasConceptScore W2624729432C86803240 @default.
• W2624729432 hasIssue "3" @default.
• W2624729432 hasLocation W26247294321 @default.
• W2624729432 hasOpenAccess W2624729432 @default.
• W2624729432 hasPrimaryLocation W26247294321 @default.
• W2624729432 hasRelatedWork W1690239356 @default.
• W2624729432 hasRelatedWork W1985467147 @default.
• W2624729432 hasRelatedWork W2008276215 @default.
• W2624729432 hasRelatedWork W2161978527 @default.
• W2624729432 hasRelatedWork W2398835055 @default.
• W2624729432 hasRelatedWork W2474990116 @default.
• W2624729432 hasRelatedWork W2811296742 @default.
• W2624729432 hasRelatedWork W2900368589 @default.
• W2624729432 hasRelatedWork W2993416897 @default.
• W2624729432 hasRelatedWork W3030814267 @default.
• W2624729432 hasRelatedWork W3030856808 @default.
• W2624729432 hasRelatedWork W3200433061 @default.
• W2624729432 hasRelatedWork W416988002 @default.
• W2624729432 hasRelatedWork W2414082154 @default.
• W2624729432 hasVolume "12" @default.
• W2624729432 isParatext "false" @default.
• W2624729432 isRetracted "false" @default.
• W2624729432 magId "2624729432" @default.
• W2624729432 workType "article" @default.