Matches in SemOpenAlex for { <https://semopenalex.org/work/W2624976498> ?p ?o ?g. }
- W2624976498 endingPage "1584" @default.
- W2624976498 startingPage "1574" @default.
- W2624976498 abstract "Osteoblast differentiation is a multistep process delicately regulated by many factors, including cytoskeletal dynamics and signaling pathways. Microtubule actin crosslinking factor 1 (MACF1), a key cytoskeletal linker, has been shown to play key roles in signal transduction and in diverse cellular processes; however, its role in regulating osteoblast differentiation is still needed to be elucidated. To further uncover the functions and mechanisms of action of MACF1 in osteoblast differentiation, we examined effects of MACF1 knockdown (MACF1-KD) in MC3T3-E1 osteoblastic cells on their osteoblast differentiation and associated molecular mechanisms. The results showed that knockdown of MACF1 significantly suppressed mineralization of MC3T3-E1 cells, down-regulated the expression of key osteogenic genes alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and type I collagen α1 (Col Iα1). Knockdown of MACF1 dramatically reduced the nuclear translocation of β-catenin, decreased the transcriptional activation of T cell factor 1 (TCF1), and down-regulated the expression of TCF1, lymphoid enhancer-binding factor 1 (LEF1), and Runx2, a target gene of β-catenin/TCF1. In addition, MACF1-KD increased the active level of glycogen synthase kinase-3β (GSK-3β), which is a key regulator for β-catenin signal transduction. Moreover, the reduction of nuclear β-catenin amount and decreased expression of TCF1 and Runx2 were significantly reversed in MACF1-KD cells when treated with lithium chloride, an agonist for β-catenin by inhibiting GSK-3β activity. Taken together, these findings suggest that knockdown of MACF1 in osteoblastic cells inhibits osteoblast differentiation through suppressing the β-catenin/TCF1-Runx2 axis. Thus, a novel role of MACF1 in and a new mechanistic insight of osteoblast differentiation are uncovered." @default.
- W2624976498 created "2017-06-23" @default.
- W2624976498 creator A5005303316 @default.
- W2624976498 creator A5015399496 @default.
- W2624976498 creator A5019311264 @default.
- W2624976498 creator A5027693027 @default.
- W2624976498 creator A5027992006 @default.
- W2624976498 creator A5030428842 @default.
- W2624976498 creator A5037883586 @default.
- W2624976498 creator A5052787170 @default.
- W2624976498 creator A5065140987 @default.
- W2624976498 creator A5070046055 @default.
- W2624976498 creator A5087287319 @default.
- W2624976498 creator A5090609386 @default.
- W2624976498 creator A5091767760 @default.
- W2624976498 date "2017-08-03" @default.
- W2624976498 modified "2023-10-16" @default.
- W2624976498 title "Microtubule actin crosslinking factor 1 promotes osteoblast differentiation by promoting β‐catenin/TCF1/Runx2 signaling axis" @default.
- W2624976498 cites W1487540566 @default.
- W2624976498 cites W1583006503 @default.
- W2624976498 cites W1980822251 @default.
- W2624976498 cites W1984489797 @default.
- W2624976498 cites W1999077006 @default.
- W2624976498 cites W2010420767 @default.
- W2624976498 cites W2018161462 @default.
- W2624976498 cites W2019196925 @default.
- W2624976498 cites W2029958661 @default.
- W2624976498 cites W2033297996 @default.
- W2624976498 cites W2037613158 @default.
- W2624976498 cites W2040644042 @default.
- W2624976498 cites W2046381945 @default.
- W2624976498 cites W2061412466 @default.
- W2624976498 cites W2071537194 @default.
- W2624976498 cites W2079141593 @default.
- W2624976498 cites W2086681436 @default.
- W2624976498 cites W2094009347 @default.
- W2624976498 cites W2107277218 @default.
- W2624976498 cites W2113442798 @default.
- W2624976498 cites W2119962765 @default.
- W2624976498 cites W2125146832 @default.
- W2624976498 cites W2133571364 @default.
- W2624976498 cites W2145300176 @default.
- W2624976498 cites W2151629140 @default.
- W2624976498 cites W2153720415 @default.
- W2624976498 cites W2159360405 @default.
- W2624976498 cites W2171483753 @default.
- W2624976498 cites W2188352508 @default.
- W2624976498 cites W2217100198 @default.
- W2624976498 cites W2314981147 @default.
- W2624976498 cites W2334589060 @default.
- W2624976498 cites W2339780974 @default.
- W2624976498 cites W2473504984 @default.
- W2624976498 cites W2534053343 @default.
- W2624976498 cites W2560648869 @default.
- W2624976498 cites W2597687928 @default.
- W2624976498 doi "https://doi.org/10.1002/jcp.26059" @default.
- W2624976498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28621459" @default.
- W2624976498 hasPublicationYear "2017" @default.
- W2624976498 type Work @default.
- W2624976498 sameAs 2624976498 @default.
- W2624976498 citedByCount "45" @default.
- W2624976498 countsByYear W26249764982017 @default.
- W2624976498 countsByYear W26249764982018 @default.
- W2624976498 countsByYear W26249764982019 @default.
- W2624976498 countsByYear W26249764982020 @default.
- W2624976498 countsByYear W26249764982021 @default.
- W2624976498 countsByYear W26249764982022 @default.
- W2624976498 countsByYear W26249764982023 @default.
- W2624976498 crossrefType "journal-article" @default.
- W2624976498 hasAuthorship W2624976498A5005303316 @default.
- W2624976498 hasAuthorship W2624976498A5015399496 @default.
- W2624976498 hasAuthorship W2624976498A5019311264 @default.
- W2624976498 hasAuthorship W2624976498A5027693027 @default.
- W2624976498 hasAuthorship W2624976498A5027992006 @default.
- W2624976498 hasAuthorship W2624976498A5030428842 @default.
- W2624976498 hasAuthorship W2624976498A5037883586 @default.
- W2624976498 hasAuthorship W2624976498A5052787170 @default.
- W2624976498 hasAuthorship W2624976498A5065140987 @default.
- W2624976498 hasAuthorship W2624976498A5070046055 @default.
- W2624976498 hasAuthorship W2624976498A5087287319 @default.
- W2624976498 hasAuthorship W2624976498A5090609386 @default.
- W2624976498 hasAuthorship W2624976498A5091767760 @default.
- W2624976498 hasConcept C104317684 @default.
- W2624976498 hasConcept C137620995 @default.
- W2624976498 hasConcept C148738053 @default.
- W2624976498 hasConcept C173396325 @default.
- W2624976498 hasConcept C185592680 @default.
- W2624976498 hasConcept C190283241 @default.
- W2624976498 hasConcept C202751555 @default.
- W2624976498 hasConcept C2776363554 @default.
- W2624976498 hasConcept C2778260815 @default.
- W2624976498 hasConcept C2779324961 @default.
- W2624976498 hasConcept C30145163 @default.
- W2624976498 hasConcept C55493867 @default.
- W2624976498 hasConcept C62478195 @default.
- W2624976498 hasConcept C86339819 @default.
- W2624976498 hasConcept C86803240 @default.
- W2624976498 hasConcept C95444343 @default.