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- W262510115 abstract "The optimum condition of rat hepatocyte primary cell culture for use as a model system to study the role of various fatty acids on cholesterol and glycerol lipid metabolism was determined. Up to 1.5 10 cells/well, a linear relationship (r = 0.96) was observed between the cell number and the quantity of cellular protein recovered after the incubation. Stearic acid bound to bovine serum albumin (BSA) at high levels in the medium (>97%) at all fatty acid-BSA ratios. Results reveal that the cellular protein (mg) can be used as the base unit to express the fatty acid metabolism in the rat hepatocyte primary culture and that stearic acid-BSA ratio of 5.6 : 1 is optimum for studying the effects of various fatty acids on lipid metabolism when considering the difficulty of handling the medium at high ratios." @default.
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- W262510115 date "2002-01-01" @default.
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- W262510115 title "Hepatocyte Primary Cell Culture as a Model System for Investigating the Effect of Stearic Acid on Cholesterol and Glycerol Lipid Metabolism" @default.
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