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- W2625626754 abstract "Enteroviruses use a type I Internal Ribosome Entry Site (IRES) structure to facilitate protein synthesis and promote genome replication. Type I IRES elements require auxiliary host proteins to organize RNA structure for 40S ribosomal subunit assembly. Heterogeneous nuclear ribonucleoprotein A1 stimulates enterovirus 71 (EV71) translation in part through specific interactions with its stem loop II (SLII) IRES domain. Here, we determined a conjoined NMR-small angle x-ray scattering structure of the EV71 SLII domain and a mutant that significantly attenuates viral replication by abrogating hnRNP A1 interactions. Native SLII adopts a locally compact structure wherein stacking interactions in a conserved 5′-AUAGC-3′ bulge preorganize the adjacent helices at nearly orthogonal orientations. Mutating the bulge sequence to 5′-ACCCC-3′ ablates base stacking in the loop and globally reorients the SLII structure. Biophysical titrations reveal that the 5′-AUAGC-3′ bulge undergoes a conformational change to assemble a functional hnRNP A1–RNA complex. Importantly, IRES mutations that delete the bulge impair viral translation and completely inhibit replication. Thus, this work provides key details into how an EV71 IRES structure adapts to hijack a cellular protein, and it suggests that the SLII domain is a potential target for antiviral therapy." @default.
- W2625626754 created "2017-06-23" @default.
- W2625626754 creator A5020175761 @default.
- W2625626754 creator A5048145620 @default.
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- W2625626754 creator A5076537379 @default.
- W2625626754 creator A5081994693 @default.
- W2625626754 creator A5089573577 @default.
- W2625626754 date "2017-09-01" @default.
- W2625626754 modified "2023-09-28" @default.
- W2625626754 title "HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation" @default.
- W2625626754 cites W1589339069 @default.
- W2625626754 cites W1766348969 @default.
- W2625626754 cites W1816304698 @default.
- W2625626754 cites W1969744746 @default.
- W2625626754 cites W1976268142 @default.
- W2625626754 cites W1977473728 @default.
- W2625626754 cites W1982126789 @default.
- W2625626754 cites W1983678802 @default.
- W2625626754 cites W1988613101 @default.
- W2625626754 cites W1991014404 @default.
- W2625626754 cites W1992191979 @default.
- W2625626754 cites W1993570118 @default.
- W2625626754 cites W1993955805 @default.
- W2625626754 cites W2003674530 @default.
- W2625626754 cites W2006158712 @default.
- W2625626754 cites W2007243503 @default.
- W2625626754 cites W2018433273 @default.
- W2625626754 cites W2021899908 @default.
- W2625626754 cites W2023590131 @default.
- W2625626754 cites W2026128357 @default.
- W2625626754 cites W2035711496 @default.
- W2625626754 cites W2043597495 @default.
- W2625626754 cites W2049915508 @default.
- W2625626754 cites W2050091361 @default.
- W2625626754 cites W2053538195 @default.
- W2625626754 cites W2062325776 @default.
- W2625626754 cites W2066088601 @default.
- W2625626754 cites W2068881880 @default.
- W2625626754 cites W2073455865 @default.
- W2625626754 cites W2076787349 @default.
- W2625626754 cites W2077401865 @default.
- W2625626754 cites W2081046982 @default.
- W2625626754 cites W2081458703 @default.
- W2625626754 cites W2082176639 @default.
- W2625626754 cites W2082800992 @default.
- W2625626754 cites W2085272777 @default.
- W2625626754 cites W2085918490 @default.
- W2625626754 cites W2092046217 @default.
- W2625626754 cites W2096567074 @default.
- W2625626754 cites W2097954103 @default.
- W2625626754 cites W2113110306 @default.
- W2625626754 cites W2116698256 @default.
- W2625626754 cites W2118233996 @default.
- W2625626754 cites W2119102790 @default.
- W2625626754 cites W2121649191 @default.
- W2625626754 cites W2122389335 @default.
- W2625626754 cites W2122732031 @default.
- W2625626754 cites W2124983865 @default.
- W2625626754 cites W2126858751 @default.
- W2625626754 cites W2127742058 @default.
- W2625626754 cites W2129168171 @default.
- W2625626754 cites W2136729627 @default.
- W2625626754 cites W2137430348 @default.
- W2625626754 cites W2141344821 @default.
- W2625626754 cites W2144794967 @default.
- W2625626754 cites W2145412955 @default.
- W2625626754 cites W2146141194 @default.
- W2625626754 cites W2149885731 @default.
- W2625626754 cites W2154714625 @default.
- W2625626754 cites W2159484613 @default.
- W2625626754 cites W2164361934 @default.
- W2625626754 cites W2166825536 @default.
- W2625626754 cites W2169821755 @default.
- W2625626754 cites W2170671445 @default.
- W2625626754 cites W2175155541 @default.
- W2625626754 cites W2219768227 @default.
- W2625626754 cites W2222794530 @default.
- W2625626754 cites W2263995572 @default.
- W2625626754 cites W2278011914 @default.
- W2625626754 cites W2288860251 @default.
- W2625626754 cites W2313116527 @default.
- W2625626754 cites W2324524889 @default.
- W2625626754 cites W2326353536 @default.
- W2625626754 cites W2332712348 @default.
- W2625626754 cites W2480071061 @default.
- W2625626754 cites W2513965901 @default.
- W2625626754 cites W2534914451 @default.
- W2625626754 cites W2536159368 @default.
- W2625626754 cites W2554920439 @default.
- W2625626754 cites W2588853197 @default.
- W2625626754 cites W259468590 @default.
- W2625626754 cites W282734715 @default.
- W2625626754 doi "https://doi.org/10.1016/j.jmb.2017.06.007" @default.
- W2625626754 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5610934" @default.
- W2625626754 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28625847" @default.
- W2625626754 hasPublicationYear "2017" @default.