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• W2626179267 abstract "Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are largely unknown. To begin to address this question, we screened for exported P. falciparum proteins that bound to the erythrocyte cytoskeleton proteins ankyrin 1 (ANK1) and band 4.1 (4.1R), which form critical interactions with other cytoskeletal proteins that contribute to the deformability and stability of RBCs. Yeast two-hybrid screens with ANK1 and 4.1R identified eight interactions with P. falciparum exported proteins, including an interaction between 4.1R and PF3D7_0402000 (PFD0090c). This interaction was first identified in a large-scale screen (Vignali et al., Malaria J, 7:211, 2008), which also reported an interaction between PF3D7_0402000 and ANK1. We confirmed the interactions of PF3D7_0402000 with 4.1R and ANK1 in pair-wise yeast two-hybrid and co-precipitation assays. In both cases, an intact PHIST domain in PF3D7_0402000 was required for binding. Complex purification followed by mass spectrometry analysis provided additional support for the interaction of PF3D7_0402000 with ANK1 and 4.1R. RBC ghost cells loaded with maltose-binding protein (MBP)-PF3D7_0402000 passed through a metal microsphere column less efficiently than mock- or MBP-loaded controls, consistent with an effect of PF3D7_0402000 on RBC rigidity or membrane stability. This study confirmed the interaction of PF3D7_0402000 with 4.1R in multiple independent assays, provided the first evidence that PF3D7_0402000 also binds to ANK1, and suggested that PF3D7_0402000 affects deformability or membrane stability of uninfected RBC ghosts." @default.
• W2626179267 created "2017-06-23" @default.
• W2626179267 creator A5037253743 @default.
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• W2626179267 date "2017-09-01" @default.
• W2626179267 modified "2023-10-10" @default.
• W2626179267 title "The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1" @default.
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• W2626179267 cites W1580396120 @default.
• W2626179267 cites W1903792504 @default.
• W2626179267 cites W1913821607 @default.
• W2626179267 cites W1967589676 @default.
• W2626179267 cites W1970072445 @default.
• W2626179267 cites W1970336017 @default.
• W2626179267 cites W1974264340 @default.
• W2626179267 cites W1982679597 @default.
• W2626179267 cites W1995447786 @default.
• W2626179267 cites W1996959443 @default.
• W2626179267 cites W1999153843 @default.
• W2626179267 cites W2003911358 @default.
• W2626179267 cites W2009052964 @default.
• W2626179267 cites W2009448974 @default.
• W2626179267 cites W2010492051 @default.
• W2626179267 cites W2018122290 @default.
• W2626179267 cites W2018447485 @default.
• W2626179267 cites W2023124677 @default.
• W2626179267 cites W2023531407 @default.
• W2626179267 cites W2024324661 @default.
• W2626179267 cites W2037042354 @default.
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• W2626179267 cites W2049729778 @default.
• W2626179267 cites W2052728471 @default.
• W2626179267 cites W2065304353 @default.
• W2626179267 cites W2075700927 @default.
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• W2626179267 cites W2110029404 @default.
• W2626179267 cites W2114662349 @default.
• W2626179267 cites W2115360771 @default.
• W2626179267 cites W2117312427 @default.
• W2626179267 cites W2122278737 @default.
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• W2626179267 cites W2164557329 @default.
• W2626179267 cites W2169966183 @default.
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• W2626179267 doi "https://doi.org/10.1016/j.molbiopara.2017.06.002" @default.
• W2626179267 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5738903" @default.
• W2626179267 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28627360" @default.
• W2626179267 hasPublicationYear "2017" @default.
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