FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2626565560> ?p ?o ?g. }

• W2626565560 endingPage "9280" @default.
• W2626565560 startingPage "9271" @default.
• W2626565560 abstract "Förster resonance energy transfer (FRET) using fluorescent base analogues is a powerful means of obtaining high-resolution nucleic acid structure and dynamics information that favorably complements techniques such as NMR and X-ray crystallography. Here, we expand the base-base FRET repertoire with an adenine analogue FRET-pair. Phosphoramidite-protected quadracyclic 2'-deoxyadenosine analogues qAN1 (donor) and qAnitro (acceptor) were synthesized and incorporated into DNA by a generic, reliable, and high-yielding route, and both constitute excellent adenine analogues. The donor, qAN1, has quantum yields reaching 21% and 11% in single- and double-strands, respectively. To the best of our knowledge, this results in the highest average brightness of an adenine analogue inside DNA. Its potent emissive features overlap well with the absorption of qAnitro and thus enable accurate FRET-measurements over more than one turn of B-DNA. As we have shown previously for our cytosine analogue FRET-pair, FRET between qAN1 and qAnitro positioned at different base separations inside DNA results in efficiencies that are highly dependent on both distance and orientation. This facilitates significantly enhanced resolution in FRET structure determinations, demonstrated here in a study of conformational changes of DNA upon binding of the minor groove binder netropsin. Finally, we note that the donor and acceptor of our cytosine FRET-pair, tCO and tCnitro, can be conveniently combined with the acceptor and donor of our current adenine pair, respectively. Consequently, our base analogues can now measure base-base FRET between 3 of the 10 possible base combinations and, through base-complementarity, between all sequence positions in a duplex." @default.
• W2626565560 created "2017-06-23" @default.
• W2626565560 creator A5024573440 @default.
• W2626565560 creator A5030474249 @default.
• W2626565560 creator A5033697022 @default.
• W2626565560 creator A5050663267 @default.
• W2626565560 creator A5061533495 @default.
• W2626565560 creator A5068579148 @default.
• W2626565560 creator A5072308039 @default.
• W2626565560 creator A5074565235 @default.
• W2626565560 creator A5081339537 @default.
• W2626565560 date "2017-06-27" @default.
• W2626565560 modified "2023-10-18" @default.
• W2626565560 title "Toward Complete Sequence Flexibility of Nucleic Acid Base Analogue FRET" @default.
• W2626565560 cites W107040987 @default.
• W2626565560 cites W1157840896 @default.
• W2626565560 cites W1579716589 @default.
• W2626565560 cites W1967121834 @default.
• W2626565560 cites W1969509102 @default.
• W2626565560 cites W1972622825 @default.
• W2626565560 cites W1973255776 @default.
• W2626565560 cites W1979270194 @default.
• W2626565560 cites W1979545166 @default.
• W2626565560 cites W1983448492 @default.
• W2626565560 cites W1994021375 @default.
• W2626565560 cites W1994221056 @default.
• W2626565560 cites W1997094705 @default.
• W2626565560 cites W1999814206 @default.
• W2626565560 cites W2002091803 @default.
• W2626565560 cites W2009771834 @default.
• W2626565560 cites W2013530233 @default.
• W2626565560 cites W2018996043 @default.
• W2626565560 cites W2023289128 @default.
• W2626565560 cites W2036229671 @default.
• W2626565560 cites W2037017264 @default.
• W2626565560 cites W2038271017 @default.
• W2626565560 cites W2054358220 @default.
• W2626565560 cites W2056476654 @default.
• W2626565560 cites W2057666290 @default.
• W2626565560 cites W2065375318 @default.
• W2626565560 cites W2066703244 @default.
• W2626565560 cites W2067878769 @default.
• W2626565560 cites W2074264340 @default.
• W2626565560 cites W2074359011 @default.
• W2626565560 cites W2074898729 @default.
• W2626565560 cites W2079650265 @default.
• W2626565560 cites W2080583450 @default.
• W2626565560 cites W2080866375 @default.
• W2626565560 cites W2080897955 @default.
• W2626565560 cites W2081397502 @default.
• W2626565560 cites W2085393878 @default.
• W2626565560 cites W2088376685 @default.
• W2626565560 cites W2094146192 @default.
• W2626565560 cites W2106452529 @default.
• W2626565560 cites W2112568806 @default.
• W2626565560 cites W2115316940 @default.
• W2626565560 cites W2116609429 @default.
• W2626565560 cites W2117226853 @default.
• W2626565560 cites W2120687754 @default.
• W2626565560 cites W2122389335 @default.
• W2626565560 cites W2123630705 @default.
• W2626565560 cites W2126330691 @default.
• W2626565560 cites W2126342106 @default.
• W2626565560 cites W2127068865 @default.
• W2626565560 cites W2132677630 @default.
• W2626565560 cites W2142844453 @default.
• W2626565560 cites W2143785581 @default.
• W2626565560 cites W2145325210 @default.
• W2626565560 cites W2150955135 @default.
• W2626565560 cites W2158896141 @default.
• W2626565560 cites W2161169616 @default.
• W2626565560 cites W2167482305 @default.
• W2626565560 cites W2168919966 @default.
• W2626565560 cites W2168921143 @default.
• W2626565560 cites W2287980474 @default.
• W2626565560 cites W2329820975 @default.
• W2626565560 cites W2434986968 @default.
• W2626565560 cites W2472776012 @default.
• W2626565560 cites W2491458126 @default.
• W2626565560 cites W2950780848 @default.
• W2626565560 cites W2951605818 @default.
• W2626565560 cites W2951649327 @default.
• W2626565560 cites W4212831456 @default.
• W2626565560 doi "https://doi.org/10.1021/jacs.7b04517" @default.
• W2626565560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28613885" @default.
• W2626565560 hasPublicationYear "2017" @default.
• W2626565560 type Work @default.
• W2626565560 sameAs 2626565560 @default.
• W2626565560 citedByCount "43" @default.
• W2626565560 countsByYear W26265655602018 @default.
• W2626565560 countsByYear W26265655602019 @default.
• W2626565560 countsByYear W26265655602020 @default.
• W2626565560 countsByYear W26265655602021 @default.
• W2626565560 countsByYear W26265655602022 @default.
• W2626565560 countsByYear W26265655602023 @default.
• W2626565560 crossrefType "journal-article" @default.
• W2626565560 hasAuthorship W2626565560A5024573440 @default.
• W2626565560 hasAuthorship W2626565560A5030474249 @default.

• next