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- W2626793756 endingPage "179" @default.
- W2626793756 startingPage "161" @default.
- W2626793756 abstract "As the field of biomaterials has expanded, it has become clear that mechanical support and diffusion is important, but not entirely sufficient, for cell proliferation and differentiation. Biomaterials also need to mimic interactions that cells experience in their natural environment. One approach is to create customized materials that interact with and modulate cell surface receptors through protein ligands. Natural proteins, however, are often not optimal for use in biomaterials, and can lack thermal, proteolytic, and chemical stability or biological specificity and potency. In addition, such proteins are often difficult to produce recombinantly, and attachment to materials can inactivate or reduce their activity. In this chapter, we provide an overview of the two main protein engineering approaches that can be used to address these limitations: rational and combinatorial protein engineering. Rational engineering requires some knowledge of a protein's structure and function, but can enable facile and effective protein modifications through site-directed mutagenesis, protein fusions, or posttranslational modifications. Alternatively, combinatorial engineering, also known as directed evolution, involves expression of a diverse library of protein mutants and screening for desired phenotypes. Techniques for both rational and combinatorial protein engineering are described. In addition, specific considerations and challenges in creating designer proteins for biomaterial applications are also discussed." @default.
- W2626793756 created "2017-06-23" @default.
- W2626793756 creator A5029326728 @default.
- W2626793756 creator A5058204963 @default.
- W2626793756 date "2011-01-01" @default.
- W2626793756 modified "2023-09-27" @default.
- W2626793756 title "Rational and Combinatorial Methods to Create Designer Protein Interfaces" @default.
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