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• W262951858 abstract "Vitamin A (C20H30O) is an essential fat-soluble vitamin for food producing animals, including sheep. Green feeds are the major source of carotenoids, including β-carotene, which in turn is metabolized to vitamin A by epithelial cells of the small intestine, which is then mainly stored in the liver. Vitamin A has many physiological functions in different tissues (1) and may provide protection against infectious diseases by enhancing many facets of the immune system. The normal value for serum vitamin A ranges from 25 to 60 µg/dl (2). The present study was carried out to determine the normal values of vitamin A and β-carotene in the blood serum and liver of Ghezel sheep in the Tabriz area of Iran. The study included 1000 Ghezel sheep that were sampled in an abattoir located in Tabriz, northwest Iran. The animals were 1-5 years old. The sheep were from flocks that grazed on range lands in the spring, summer, and the first half of autumn. They ate sun-cured harvested forage and some barley grain as concentrate when they were in the corral, until the end of March. Samples of whole blood (5 ml) and liver tissue (5 g) were obtained before and after slaughter. Vitamin A and β-carotene levels of the samples were determined spectrophotometrically (3). Briefly, 1 ml of serum was pipetted into a centrifuge tube, followed by the addition of 1 ml of 95% ethanolic KOH and 1.5 ml of hexane. The mixture was stoppered and vortexed vigorously for 2 min and centrifuged at 3000 × g for 10 min. The upper hexane extract (2 ml) was transferred into a dry test tube and the absorbance of the extract was read at 325 nm against a hexane blank using a Biowave spectrophotometer. Then, the absorbance of the extract was read again at 453 nm against a hexane blank. For assaying the liver concentrations of vitamin A and β-carotene, 1 g of liver was crushed in a mortar and mixed with 10 ml of ethanol. From this, a 1-ml aliquot was taken for analysis, using the serum assay. The concentrations of vitamin A and β-carotene in the serum (µg/dl) and liver (µg/g) were calculated using the formulae: )" @default.
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• W262951858 date "2008-02-01" @default.
• W262951858 modified "2023-10-14" @default.
• W262951858 title "Seasonal Variation of Vitamin A and Beta-Carotene Levels in Ghezel Sheep" @default.
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