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- W263027968 abstract "The first cytochrome to be described in the chloroplasts was cytochrome f, which has been shown to be of the c type. It is recommended that the chloroplasts be obtained in the intact state from the leaf. This is best accomplished by a very short grinding or blending of the leaf material—this and all subsequent processes are to be carried out below 5°. The leaf material is preferably freed from the main leaf veins and kept in a turgid condition. By using sucrose or other neutral carbohydrates to adjust the osmotic pressure, rather than salt, the chloroplast preparation can be obtained substantially free from other cytoplasmic organelles—this may be checked by suitable microscopic examination. In spite of the universal occurrence of cytochrome f in higher-plant chloroplasts the only highly purified soluble preparations to be described have been obtained from leaves of curled parsley. Because cytochrome f is confined to chloroplasts, it would appear advantageous that the starting material should be isolated chloroplasts or their fragments freed by washing from cytoplasmic proteins, soluble chloroplast proteins, vacuolar acids, and phenolics. Ammoniacal ethanol, however, extracts insignificant amounts of the cytochrome from such material. The chapter describes the procedure for the purification of the extracted cytochrome." @default.
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- W263027968 date "1971-01-01" @default.
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- W263027968 title "[33] Cytochrome components in chloroplasts of the higher plants" @default.
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