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• W2632796145 abstract "Chitosan scaffolds of different deacetylation degrees, average molecular weights and concentrations reinforced with silica nanoparticles were prepared for bone tissue regeneration. The resulting nanocomposites showed similar pore sizes (<300 μm) regardless the deacetylation degree and concentration used in their formulation. Their mechanical compression resistance was increased by a 30% with the addition of silica nanoparticles as nanofillers. The biocompatibility of the three-dimensional chitosan scaffolds was confirmed by the Alamar Blue assay in human primary osteoblasts as well as the formation of cell spheroids indicative of their great potential for bone regeneration. In vivo implantation of the scaffolds in a mice calvaria defect model provided substantial evidences of the suitability of these nanocomposites for bone tissue engineering showing a mature and dense collagenous tissue with small foci of mineralization, vascularized areas and the infiltration of osteoblasts and osteoclasts. Nevertheless, mature bone tissue formation was not observed after eight weeks of implantation." @default.
• W2632796145 created "2017-06-30" @default.
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• W2632796145 date "2017-10-01" @default.
• W2632796145 modified "2023-10-03" @default.
• W2632796145 title "Chitosan-based nanocomposites for the repair of bone defects" @default.
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• W2632796145 doi "https://doi.org/10.1016/j.nano.2017.06.007" @default.
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