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• W2634408610 endingPage "e1006456" @default.
• W2634408610 startingPage "e1006456" @default.
• W2634408610 abstract "5’-3’ decay is the major mRNA decay pathway in many eukaryotes, including trypanosomes. After deadenylation, mRNAs are decapped by the nudix hydrolase DCP2 of the decapping complex and finally degraded by the 5’-3’ exoribonuclease. Uniquely, trypanosomes lack homologues to all subunits of the decapping complex, while deadenylation and 5’-3’ degradation are conserved. Here, I show that the parasites use an ApaH-like phosphatase (ALPH1) as their major mRNA decapping enzyme. The protein was recently identified as a novel trypanosome stress granule protein and as involved in mRNA binding. A fraction of ALPH1 co-localises exclusively with the trypanosome 5’-3’ exoribonuclease XRNA to a special granule at the posterior pole of the cell, indicating a connection between the two enzymes. RNAi depletion of ALPH1 is lethal and causes a massive increase in total mRNAs that are deadenylated, but have not yet started 5’-3’ decay. These data suggest that ALPH1 acts downstream of deadenylation and upstream of mRNA degradation, consistent with a function in mRNA decapping. In vitro experiments show that recombinant, N-terminally truncated ALHP1 protein, but not a catalytically inactive mutant, sensitises the capped trypanosome spliced leader RNA to yeast Xrn1, but only if an RNA 5’ polyphosphatase is included. This indicates that the decapping mechanism of ALPH1 differs from the decapping mechanism of Dcp2 by leaving more than one phosphate group at the mRNA’s 5’ end. This is the first reported function of a eukaryotic ApaH-like phosphatase, a bacterial-derived class of enzymes present in all phylogenetic super-groups of the eukaryotic kingdom. The substrates of eukaryotic ApaH-like phosphatases are unknown. However, the substrate of the related bacterial enzyme ApaH, diadenosine tetraphosphate, is highly reminiscent of a eukaryotic mRNA cap." @default.
• W2634408610 created "2017-06-30" @default.
• W2634408610 creator A5091728303 @default.
• W2634408610 date "2017-06-19" @default.
• W2634408610 modified "2023-10-16" @default.
• W2634408610 title "The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes" @default.
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• W2634408610 doi "https://doi.org/10.1371/journal.ppat.1006456" @default.
• W2634408610 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5491325" @default.
• W2634408610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28628654" @default.
• W2634408610 hasPublicationYear "2017" @default.
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