FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2653315286> ?p ?o ?g. }

• W2653315286 abstract "Hepatitis C virus (HCV) induces the expression of the genes of proinflammatory cytokines, the excessive production of which may cause cell death, and contribute to development of liver fibrosis and hepatocarcinoma. The relationship between cytokine production and metabolic disorders in HCV-infected cells remains obscure. The levels of biogenic polyamines, spermine, spermidine, and their precursor putrescine, may be a potential regulator of these processes. The purpose of the present work was to study the effects of the compounds which modulate biogenic polyamines metabolism on cytokine production and HCV proteins expression. Human hepatocarcinoma Huh7.5 cells have been transfected with the plasmids that encode HCV proteins and further incubated with the following low-molecular compounds that affect different stages of polyamine metabolism: (1) difluoromethylornithine (DFMO), the inhibitor of ornithine decarboxylase, the enzyme that catalyzes the biosynthesis of polyamines; (2) N,N'-bis(2,3-butane dienyl)-1,4-diaminobutane (MDL72.527), the inhibitor of proteins involved in polyamine degradation; and (3) synthetic polyamine analog N^(I),N^(II)-diethylnorspermine (DENSpm), an inducer of polyamine degradation enzyme. The intracellular accumulation and secretion of cytokines (IL-6, IL-1β, TNF-α, and TGF-β) was assessed by immunocytochemistry and in the immunoenzyme assay, while the cytokine gene expression was studied using reverse transcription and PCR. The effects of the compounds under analysis on the expression of HCV proteins were analyzed using the indirect immunofluorescence with anti-HCV monoclonal antibodies. It has been demonstrated that, in cells transfected with HCV genes, DFMO reduces the production of three out of four tested cytokines, namely, TNF-α and TGF-β in cells that express HCV core, Е1Е2, NS3, NS5A, and NS5B proteins, and IL-1β in the cells that express HCV core, Е1Е2, and NS3 proteins. MDL72527 and DENSpm decreased cytokine production to a lesser extent. Incubation with DFMO led to a 28-32% decrease in the number of cells expressing NS5B or NS5A, both of which are key components of the HCV replication complex. The results obtained in the work indicate that a further detailed study of the antiviral activity of DFMO is required in order to assess its potential as an anti-hepatitis C therapeutic agent." @default.
• W2653315286 created "2017-06-30" @default.
• W2653315286 creator A5014873443 @default.
• W2653315286 creator A5017383763 @default.
• W2653315286 creator A5031130036 @default.
• W2653315286 creator A5044978887 @default.
• W2653315286 creator A5045501920 @default.
• W2653315286 creator A5083290696 @default.
• W2653315286 creator A5087488652 @default.
• W2653315286 date "2017-01-01" @default.
• W2653315286 modified "2023-09-24" @default.
• W2653315286 title "Низкомолекулярные регуляторы метаболизма биогенных полиаминов влияют на продукцию цитокинов и экспрессию белков вируса гепатита С в клетках гепатокарциномы человека Huh7.5, Молекулярная биология" @default.
• W2653315286 cites W1523148799 @default.
• W2653315286 cites W1523727431 @default.
• W2653315286 cites W1547594908 @default.
• W2653315286 cites W1723554443 @default.
• W2653315286 cites W183377719 @default.
• W2653315286 cites W1963552057 @default.
• W2653315286 cites W1971884054 @default.
• W2653315286 cites W1981513035 @default.
• W2653315286 cites W2003579943 @default.
• W2653315286 cites W2014830013 @default.
• W2653315286 cites W2028766649 @default.
• W2653315286 cites W2031004199 @default.
• W2653315286 cites W2042248963 @default.
• W2653315286 cites W2043198295 @default.
• W2653315286 cites W2059246129 @default.
• W2653315286 cites W2059420020 @default.
• W2653315286 cites W2067673857 @default.
• W2653315286 cites W2067852189 @default.
• W2653315286 cites W2079193866 @default.
• W2653315286 cites W2087821048 @default.
• W2653315286 cites W2114267277 @default.
• W2653315286 cites W2120125627 @default.
• W2653315286 cites W2136354950 @default.
• W2653315286 cites W2147126871 @default.
• W2653315286 cites W2147621980 @default.
• W2653315286 cites W2154951555 @default.
• W2653315286 cites W2157650174 @default.
• W2653315286 cites W2165772492 @default.
• W2653315286 cites W2232785457 @default.
• W2653315286 cites W2327943723 @default.
• W2653315286 cites W2337811567 @default.
• W2653315286 doi "https://doi.org/10.7868/s0026898417030120" @default.
• W2653315286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28707668" @default.
• W2653315286 hasPublicationYear "2017" @default.
• W2653315286 type Work @default.
• W2653315286 sameAs 2653315286 @default.
• W2653315286 citedByCount "1" @default.
• W2653315286 countsByYear W26533152862020 @default.
• W2653315286 crossrefType "journal-article" @default.
• W2653315286 hasAuthorship W2653315286A5014873443 @default.
• W2653315286 hasAuthorship W2653315286A5017383763 @default.
• W2653315286 hasAuthorship W2653315286A5031130036 @default.
• W2653315286 hasAuthorship W2653315286A5044978887 @default.
• W2653315286 hasAuthorship W2653315286A5045501920 @default.
• W2653315286 hasAuthorship W2653315286A5083290696 @default.
• W2653315286 hasAuthorship W2653315286A5087488652 @default.
• W2653315286 hasConcept C104317684 @default.
• W2653315286 hasConcept C121289238 @default.
• W2653315286 hasConcept C153911025 @default.
• W2653315286 hasConcept C164027704 @default.
• W2653315286 hasConcept C181199279 @default.
• W2653315286 hasConcept C203014093 @default.
• W2653315286 hasConcept C2776109719 @default.
• W2653315286 hasConcept C2776914184 @default.
• W2653315286 hasConcept C2777167505 @default.
• W2653315286 hasConcept C2777497464 @default.
• W2653315286 hasConcept C2778690821 @default.
• W2653315286 hasConcept C2780115381 @default.
• W2653315286 hasConcept C54009773 @default.
• W2653315286 hasConcept C55493867 @default.
• W2653315286 hasConcept C86803240 @default.
• W2653315286 hasConceptScore W2653315286C104317684 @default.
• W2653315286 hasConceptScore W2653315286C121289238 @default.
• W2653315286 hasConceptScore W2653315286C153911025 @default.
• W2653315286 hasConceptScore W2653315286C164027704 @default.
• W2653315286 hasConceptScore W2653315286C181199279 @default.
• W2653315286 hasConceptScore W2653315286C203014093 @default.
• W2653315286 hasConceptScore W2653315286C2776109719 @default.
• W2653315286 hasConceptScore W2653315286C2776914184 @default.
• W2653315286 hasConceptScore W2653315286C2777167505 @default.
• W2653315286 hasConceptScore W2653315286C2777497464 @default.
• W2653315286 hasConceptScore W2653315286C2778690821 @default.
• W2653315286 hasConceptScore W2653315286C2780115381 @default.
• W2653315286 hasConceptScore W2653315286C54009773 @default.
• W2653315286 hasConceptScore W2653315286C55493867 @default.
• W2653315286 hasConceptScore W2653315286C86803240 @default.
• W2653315286 hasLocation W26533152861 @default.
• W2653315286 hasLocation W26533152862 @default.
• W2653315286 hasOpenAccess W2653315286 @default.
• W2653315286 hasPrimaryLocation W26533152861 @default.
• W2653315286 hasRelatedWork W1494632330 @default.
• W2653315286 hasRelatedWork W2020898100 @default.
• W2653315286 hasRelatedWork W2034854973 @default.
• W2653315286 hasRelatedWork W2044947009 @default.
• W2653315286 hasRelatedWork W2060591613 @default.
• W2653315286 hasRelatedWork W2083729530 @default.
• W2653315286 hasRelatedWork W2188247425 @default.
• W2653315286 hasRelatedWork W2414326766 @default.

• next