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- W266552930 abstract "Aim To accomplish the etiological diagnosis of toxoplasmosis by in situ PCR(ISPCR) Methods Mice inoculated with 2×10 2 tachyzoites of Toxoplasma gondii(RH strain)were sacrificed 2、4、6 days after infection The livers were taken out and prepared as paraffin embedded sections and frozen sections Direct ISPCR was employed to amplify and detect the DNA of T gondii in paraffin embedded sections The results were compared with that of immunohistochemical detection in frozen sections Results DNA of T gondii were detected Form All the 18 secxions by using ISPCR,while(11/18)were positive by immunohistochemical detection Conclusions ISPCR could be applicable in detecting the DNA of T gondii in paraffin embedded sections as a new method" @default.
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- W266552930 date "2000-01-01" @default.
- W266552930 modified "2023-09-24" @default.
- W266552930 title "Application of in situ PCR in the pathological detection of toxoplasmosis." @default.
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