Matches in SemOpenAlex for { <https://semopenalex.org/work/W2666656995> ?p ?o ?g. }
- W2666656995 endingPage "1347" @default.
- W2666656995 startingPage "1339" @default.
- W2666656995 abstract "Purpose: To explore the inhibitory activity of Lactobacillus salivarius ssp. salivarius JCM1231 (L. salivarius JCM1231) culture filtrate against Fusarium solani (F. solani) and its effects on murine keratocytes (MKs) infected with F. solani.Methods: L. salivarius JCM1231 was cultured in an anaerobic incubator for 24 h, and the L. salivarius culture filtrate (LSCF) was prepared .The antifungal activity of L. salivarius JCM1231 against F. solani was determined with a plate overlay assay, agar diffusion assay, and conidial germination inhibition test. The effects of temperature, pH, and proteolytic enzymes on the antifungal activity of LSCF were detected with microtiter plate-well assay and conidial germination inhibition assay. Furthermore, the effects of LSCF on MKs infected with F. solani were detected. Cell activity and apoptosis were measured using methylthiazoletetrazolium assays and flow cytometry analysis, respectively. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) cytokines were measured using real-time polymerase chain reactions and enzyme-linked immunosorbent assays (ELISA), and mycotoxin production was detected with high-performance liquid chromatography tandem mass spectrometry.Results: Conidial germination and mycelia growth of F. solani were significantly inhibited by LSCF. The antifungal substances produced by L. salivarius JCM1231 were heat unstable, proteinaceous, and sensitive to proteolytic enzymes and were active within a narrow acidic pH range between 2.0 and 4.0. In the presence of 15 µg/ml of LSCF, cell activity was significantly increased, and cell apoptosis, the level of IL-6 and TNF-α expressions, and mycotoxin (zearalenone and fumonisin B1) productions were decreased significantly in MKs infected with F. solani.Conclusion: L. salivarius JCM1231 culture filtrate can effectively inhibit F. solani growth and protect MKs against F. solani infection." @default.
- W2666656995 created "2017-06-30" @default.
- W2666656995 creator A5022634388 @default.
- W2666656995 creator A5041180523 @default.
- W2666656995 creator A5051364953 @default.
- W2666656995 creator A5053647655 @default.
- W2666656995 creator A5074040550 @default.
- W2666656995 creator A5082859611 @default.
- W2666656995 date "2017-06-21" @default.
- W2666656995 modified "2023-09-24" @default.
- W2666656995 title "Inhibition of <i>Fusarium solani</i> Infection in Murine Keratocytes by <i>Lactobacillus salivarius</i> ssp. <i>salivarius JCM1231</i> Culture Filtrate In Vitro" @default.
- W2666656995 cites W1845752854 @default.
- W2666656995 cites W1903365558 @default.
- W2666656995 cites W1968347085 @default.
- W2666656995 cites W1968580186 @default.
- W2666656995 cites W1979205933 @default.
- W2666656995 cites W1983370139 @default.
- W2666656995 cites W1989188055 @default.
- W2666656995 cites W2008885970 @default.
- W2666656995 cites W2017569060 @default.
- W2666656995 cites W2020180681 @default.
- W2666656995 cites W2023845464 @default.
- W2666656995 cites W2034611781 @default.
- W2666656995 cites W2040308872 @default.
- W2666656995 cites W2042370398 @default.
- W2666656995 cites W2060502769 @default.
- W2666656995 cites W2061797039 @default.
- W2666656995 cites W2062472092 @default.
- W2666656995 cites W2065956443 @default.
- W2666656995 cites W2066031308 @default.
- W2666656995 cites W2070496611 @default.
- W2666656995 cites W2075945132 @default.
- W2666656995 cites W2081618734 @default.
- W2666656995 cites W2083792425 @default.
- W2666656995 cites W2103472692 @default.
- W2666656995 cites W2118844553 @default.
- W2666656995 cites W2128101715 @default.
- W2666656995 cites W2129131406 @default.
- W2666656995 cites W2130927881 @default.
- W2666656995 cites W2134033832 @default.
- W2666656995 cites W2139540667 @default.
- W2666656995 cites W2140130533 @default.
- W2666656995 cites W2149404457 @default.
- W2666656995 cites W2151993668 @default.
- W2666656995 cites W2153077179 @default.
- W2666656995 cites W2166890422 @default.
- W2666656995 cites W2167959540 @default.
- W2666656995 cites W2170740826 @default.
- W2666656995 doi "https://doi.org/10.1080/02713683.2017.1317816" @default.
- W2666656995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28636459" @default.
- W2666656995 hasPublicationYear "2017" @default.
- W2666656995 type Work @default.
- W2666656995 sameAs 2666656995 @default.
- W2666656995 citedByCount "4" @default.
- W2666656995 countsByYear W26666569952019 @default.
- W2666656995 countsByYear W26666569952022 @default.
- W2666656995 countsByYear W26666569952023 @default.
- W2666656995 crossrefType "journal-article" @default.
- W2666656995 hasAuthorship W2666656995A5022634388 @default.
- W2666656995 hasAuthorship W2666656995A5041180523 @default.
- W2666656995 hasAuthorship W2666656995A5051364953 @default.
- W2666656995 hasAuthorship W2666656995A5053647655 @default.
- W2666656995 hasAuthorship W2666656995A5074040550 @default.
- W2666656995 hasAuthorship W2666656995A5082859611 @default.
- W2666656995 hasConcept C100544194 @default.
- W2666656995 hasConcept C133479454 @default.
- W2666656995 hasConcept C185592680 @default.
- W2666656995 hasConcept C2780206646 @default.
- W2666656995 hasConcept C2780795924 @default.
- W2666656995 hasConcept C31903555 @default.
- W2666656995 hasConcept C59822182 @default.
- W2666656995 hasConcept C86803240 @default.
- W2666656995 hasConcept C89423630 @default.
- W2666656995 hasConceptScore W2666656995C100544194 @default.
- W2666656995 hasConceptScore W2666656995C133479454 @default.
- W2666656995 hasConceptScore W2666656995C185592680 @default.
- W2666656995 hasConceptScore W2666656995C2780206646 @default.
- W2666656995 hasConceptScore W2666656995C2780795924 @default.
- W2666656995 hasConceptScore W2666656995C31903555 @default.
- W2666656995 hasConceptScore W2666656995C59822182 @default.
- W2666656995 hasConceptScore W2666656995C86803240 @default.
- W2666656995 hasConceptScore W2666656995C89423630 @default.
- W2666656995 hasFunder F4320321001 @default.
- W2666656995 hasIssue "10" @default.
- W2666656995 hasLocation W26666569951 @default.
- W2666656995 hasLocation W26666569952 @default.
- W2666656995 hasOpenAccess W2666656995 @default.
- W2666656995 hasPrimaryLocation W26666569951 @default.
- W2666656995 hasRelatedWork W1975301516 @default.
- W2666656995 hasRelatedWork W2020275038 @default.
- W2666656995 hasRelatedWork W2091219986 @default.
- W2666656995 hasRelatedWork W2096078730 @default.
- W2666656995 hasRelatedWork W2106146882 @default.
- W2666656995 hasRelatedWork W2892743138 @default.
- W2666656995 hasRelatedWork W2915028625 @default.
- W2666656995 hasRelatedWork W4241306252 @default.
- W2666656995 hasRelatedWork W4254314521 @default.
- W2666656995 hasRelatedWork W4309008726 @default.