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- W266988172 abstract "The D10G4.1 T cell line has a receptor for I-A/sup k/ and conalbumin. The monoclonal mouse antibody 3D3 binds to and activates this receptor. In the presence of either IL-1 or IL-2, and 3D3 antibody, D10 cells are stimulated to divide. If 3D3 antibody was added to the cells first, IL-1 or IL-2 could be added up to 12 hours later, with only about 45% decrease in response as measured by tritiated thymidine (/sup 3/HTdR) uptake at 72 hours. Similarly, if IL-2 was added first, the addition of 3D3 antibody could be delayed as much as 12 hours with only slight decline (18%) in /sup 3/HTdR uptake. However, if the cells were exposed to IL-1 for only two hours before the addition of 3D3, the response was reduced by 76%, and if 3D3 addition was delayed to 12 hours, the response was reduced by 95%. These results suggest that exposure of D10 cells to IL-1 alone, in the absence of an antigen stimulus strongly inhibits the subsequent response to antigen." @default.
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- W266988172 date "1986-03-05" @default.
- W266988172 modified "2023-09-24" @default.
- W266988172 title "Effects of asynchronous stimulation of D10G4. 1 cells with 3D3 monoclonal antibody, and interleukin-1 (IL-1) or interleukin-2 (IL-2)" @default.
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