FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2676635625> ?p ?o ?g. }

• W2676635625 endingPage "7709" @default.
• W2676635625 startingPage "7696" @default.
• W2676635625 abstract "The ratio of different AA in the diets of cows is vital to improve milk protein yield. β-Casein is one of the important milk proteins with high nutritive value. However, the suitable ratio of essential amino acids (EAA) for the expression of β-casein in the immortalized bovine mammary epithelial cell line is not fully characterized. This study employed response surface methodology to determine the optimal ratio of His, Lys, Met, and Leu on β-casein expression level in vitro and clarified the effect of the 4 EAA on β-casein via the mechanistic target of rapamycin (mTOR) signaling pathway. A central composite design containing 5 axial points per EAA and 28 combinations of the 4 EAA was used in our study. The results of response surface methodology and the changes of the mTOR-related signaling proteins were determined by western blot. The results showed that β-casein level was significantly affected by all 4 EAA (R2 = 0.71). The optimum conditions for β-casein expression are found to be 5.47 mM of His, 7.48 mM of Lys, 1.17 mM of Met, and 8.21 mM of Leu (His:Lys:Met:Leu = 5:6:1:7) in the designed scope of concentration. The interaction of Leu and Met significantly affected β-casein expression (P < 0.01). The phosphorylation of mTOR (Ser2481), regulatory associated protein of target of rapamycin (Ser792), ribosomal protein S6 kinase 1 (Thr389), ribosomal protein S6 (Ser235/236), and eukaryotic elongation factor 2 (Thr56) was increased with the supplementation of either single EAA or an optimal combination of EAA. However, the phosphorylation of eukaryotic initiation factor 4E binding protein 1 (Thr37) was decreased with the addition of Lys, Met, or Leu alone. Furthermore, the phosphorylation (P) of eIF2α (Ser51) was decreased when Met was supplemented alone. Under the optimal mixture of 4 EAA, the phosphorylation of mechanistic target of rapamycin complex 1 signaling proteins was significantly greater than the single EAA supplementations and the expression of β-casein was 98% as high as the positive control (i.e., medium with all AA). A similar trend was found with P-ribosomal protein S6 kinase 1 and P-ribosomal protein S6. In conclusion, the extracellular concentrations of His, Lys, Met, and Leu at a ratio of 5:6:1:7 maximized β-casein expression in the immortalized bovine mammary epithelial cell line may occur via activation of the mechanistic target of rapamycin complex 1 signaling pathway." @default.
• W2676635625 created "2017-06-30" @default.
• W2676635625 creator A5006405451 @default.
• W2676635625 creator A5040456958 @default.
• W2676635625 creator A5045659613 @default.
• W2676635625 creator A5065660628 @default.
• W2676635625 creator A5075662602 @default.
• W2676635625 creator A5089048824 @default.
• W2676635625 creator A5089255910 @default.
• W2676635625 date "2017-09-01" @default.
• W2676635625 modified "2023-10-17" @default.
• W2676635625 title "Combination of histidine, lysine, methionine, and leucine promotes β-casein synthesis via the mechanistic target of rapamycin signaling pathway in bovine mammary epithelial cells" @default.
• W2676635625 cites W1737152983 @default.
• W2676635625 cites W1873073107 @default.
• W2676635625 cites W1902636525 @default.
• W2676635625 cites W1959856935 @default.
• W2676635625 cites W1964188972 @default.
• W2676635625 cites W1990909031 @default.
• W2676635625 cites W1994206543 @default.
• W2676635625 cites W2003190363 @default.
• W2676635625 cites W2005251123 @default.
• W2676635625 cites W2006559495 @default.
• W2676635625 cites W2007870253 @default.
• W2676635625 cites W2023012828 @default.
• W2676635625 cites W2023693413 @default.
• W2676635625 cites W2023729042 @default.
• W2676635625 cites W2028272493 @default.
• W2676635625 cites W2035205542 @default.
• W2676635625 cites W2038086056 @default.
• W2676635625 cites W2042353023 @default.
• W2676635625 cites W2042894701 @default.
• W2676635625 cites W2044889844 @default.
• W2676635625 cites W2044958613 @default.
• W2676635625 cites W2047317002 @default.
• W2676635625 cites W2048498664 @default.
• W2676635625 cites W2051652691 @default.
• W2676635625 cites W2059222888 @default.
• W2676635625 cites W2059515698 @default.
• W2676635625 cites W2064627937 @default.
• W2676635625 cites W2074645484 @default.
• W2676635625 cites W2079186013 @default.
• W2676635625 cites W2088927005 @default.
• W2676635625 cites W2098869037 @default.
• W2676635625 cites W2098989716 @default.
• W2676635625 cites W2100936359 @default.
• W2676635625 cites W2113697770 @default.
• W2676635625 cites W2117912846 @default.
• W2676635625 cites W2123231624 @default.
• W2676635625 cites W2127848114 @default.
• W2676635625 cites W2128551987 @default.
• W2676635625 cites W2129260297 @default.
• W2676635625 cites W2132531320 @default.
• W2676635625 cites W2133684121 @default.
• W2676635625 cites W2144059623 @default.
• W2676635625 cites W2164420978 @default.
• W2676635625 cites W2167376319 @default.
• W2676635625 cites W2170361722 @default.
• W2676635625 cites W2415488515 @default.
• W2676635625 cites W4252765228 @default.
• W2676635625 doi "https://doi.org/10.3168/jds.2015-10729" @default.
• W2676635625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28647331" @default.
• W2676635625 hasPublicationYear "2017" @default.
• W2676635625 type Work @default.
• W2676635625 sameAs 2676635625 @default.
• W2676635625 citedByCount "21" @default.
• W2676635625 countsByYear W26766356252018 @default.
• W2676635625 countsByYear W26766356252019 @default.
• W2676635625 countsByYear W26766356252020 @default.
• W2676635625 countsByYear W26766356252021 @default.
• W2676635625 countsByYear W26766356252022 @default.
• W2676635625 countsByYear W26766356252023 @default.
• W2676635625 crossrefType "journal-article" @default.
• W2676635625 hasAuthorship W2676635625A5006405451 @default.
• W2676635625 hasAuthorship W2676635625A5040456958 @default.
• W2676635625 hasAuthorship W2676635625A5045659613 @default.
• W2676635625 hasAuthorship W2676635625A5065660628 @default.
• W2676635625 hasAuthorship W2676635625A5075662602 @default.
• W2676635625 hasAuthorship W2676635625A5089048824 @default.
• W2676635625 hasAuthorship W2676635625A5089255910 @default.
• W2676635625 hasBestOaLocation W26766356251 @default.
• W2676635625 hasConcept C104202773 @default.
• W2676635625 hasConcept C104317684 @default.
• W2676635625 hasConcept C11960822 @default.
• W2676635625 hasConcept C143910341 @default.
• W2676635625 hasConcept C160110348 @default.
• W2676635625 hasConcept C185592680 @default.
• W2676635625 hasConcept C2776142715 @default.
• W2676635625 hasConcept C2776415932 @default.
• W2676635625 hasConcept C2776580952 @default.
• W2676635625 hasConcept C2780912031 @default.
• W2676635625 hasConcept C2780926556 @default.
• W2676635625 hasConcept C38062823 @default.
• W2676635625 hasConcept C515207424 @default.
• W2676635625 hasConcept C55493867 @default.
• W2676635625 hasConcept C62478195 @default.
• W2676635625 hasConcept C67705224 @default.
• W2676635625 hasConcept C75217442 @default.
• W2676635625 hasConcept C86554907 @default.

• next