FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2677486574> ?p ?o ?g. }

• W2677486574 abstract "ABSTRACT In chronic respiratory disease, the formation of dense, 3-dimensional “microcolonies” by Pseudomonas aeruginosa within the airway plays an important role in contributing to resistance to treatment. An in vitro biofilm model of pseudomonal microcolony formation using artificial-sputum (AS) medium was established to study the effects of low-molecular-weight alginate oligomers (OligoG CF-5/20) on pseudomonal growth, microcolony formation, and the efficacy of colistin. The studies employed clinical cystic fibrosis (CF) isolates ( n = 3) and reference nonmucoid and mucoid multidrug-resistant (MDR) CF isolates ( n = 7). Bacterial growth and biofilm development and disruption were studied using cell viability assays and image analysis with scanning electron and confocal laser scanning microscopy. Pseudomonal growth in AS medium was associated with increased ATP production ( P < 0.05) and the formation (at 48 h) of discrete (>10-μm) microcolonies. In conventional growth medium, colistin retained an ability to inhibit growth of planktonic bacteria, although the MIC was increased (0.1 to 0.4 μg/ml) in AS medium compared to Mueller-Hinton (MH) medium. In contrast, in an established-biofilm model in AS medium, the efficacy of colistin was decreased. OligoG CF-5/20 (≥2%) treatment, however, induced dose-dependent biofilm disruption ( P < 0.05) and led to colistin retaining its antimicrobial activity ( P < 0.05). While circular dichroism indicated that OligoG CF-5/20 did not change the orientation of the alginate carboxyl groups, mass spectrometry demonstrated that the oligomers induced dose-dependent (>0.2%; P < 0.05) reductions in pseudomonal quorum-sensing signaling. These findings reinforce the potential clinical significance of microcolony formation in the CF lung and highlight a novel approach to treat MDR pseudomonal infections." @default.
• W2677486574 created "2017-06-30" @default.
• W2677486574 creator A5010362194 @default.
• W2677486574 creator A5017125420 @default.
• W2677486574 creator A5019415991 @default.
• W2677486574 creator A5021855910 @default.
• W2677486574 creator A5023498981 @default.
• W2677486574 creator A5043164290 @default.
• W2677486574 creator A5051471066 @default.
• W2677486574 creator A5058637743 @default.
• W2677486574 creator A5069143016 @default.
• W2677486574 creator A5075658046 @default.
• W2677486574 creator A5076620288 @default.
• W2677486574 date "2017-09-01" @default.
• W2677486574 modified "2023-10-12" @default.
• W2677486574 title "A Low-Molecular-Weight Alginate Oligosaccharide Disrupts Pseudomonal Microcolony Formation and Enhances Antibiotic Effectiveness" @default.
• W2677486574 cites W1946658523 @default.
• W2677486574 cites W1955626095 @default.
• W2677486574 cites W1972250494 @default.
• W2677486574 cites W1972750161 @default.
• W2677486574 cites W1984005894 @default.
• W2677486574 cites W1984693521 @default.
• W2677486574 cites W1988200986 @default.
• W2677486574 cites W1988680982 @default.
• W2677486574 cites W1990364841 @default.
• W2677486574 cites W2003061612 @default.
• W2677486574 cites W2006405242 @default.
• W2677486574 cites W2031213868 @default.
• W2677486574 cites W2033607922 @default.
• W2677486574 cites W2035226835 @default.
• W2677486574 cites W2052897440 @default.
• W2677486574 cites W2053305756 @default.
• W2677486574 cites W2055117023 @default.
• W2677486574 cites W2058406790 @default.
• W2677486574 cites W2062270991 @default.
• W2677486574 cites W2062539253 @default.
• W2677486574 cites W2064543837 @default.
• W2677486574 cites W2067983977 @default.
• W2677486574 cites W2070300719 @default.
• W2677486574 cites W2070432566 @default.
• W2677486574 cites W2073824449 @default.
• W2677486574 cites W2076595398 @default.
• W2677486574 cites W2077572900 @default.
• W2677486574 cites W2078193677 @default.
• W2677486574 cites W2089190407 @default.
• W2677486574 cites W2097145546 @default.
• W2677486574 cites W2102561895 @default.
• W2677486574 cites W2106351298 @default.
• W2677486574 cites W2114846377 @default.
• W2677486574 cites W2116716923 @default.
• W2677486574 cites W2117992352 @default.
• W2677486574 cites W2121890764 @default.
• W2677486574 cites W2127778188 @default.
• W2677486574 cites W2127836223 @default.
• W2677486574 cites W2130951311 @default.
• W2677486574 cites W2132211475 @default.
• W2677486574 cites W2148357124 @default.
• W2677486574 cites W2153102800 @default.
• W2677486574 cites W2162335168 @default.
• W2677486574 cites W2163458252 @default.
• W2677486574 cites W2163803918 @default.
• W2677486574 cites W2163856333 @default.
• W2677486574 cites W2164032940 @default.
• W2677486574 cites W2170521038 @default.
• W2677486574 cites W2171632431 @default.
• W2677486574 cites W2179036997 @default.
• W2677486574 cites W2201817029 @default.
• W2677486574 cites W2254678862 @default.
• W2677486574 cites W2262251108 @default.
• W2677486574 cites W2298005996 @default.
• W2677486574 cites W2324398833 @default.
• W2677486574 cites W4252523298 @default.
• W2677486574 cites W649773320 @default.
• W2677486574 doi "https://doi.org/10.1128/aac.00762-17" @default.
• W2677486574 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5571347" @default.
• W2677486574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28630204" @default.
• W2677486574 hasPublicationYear "2017" @default.
• W2677486574 type Work @default.
• W2677486574 sameAs 2677486574 @default.
• W2677486574 citedByCount "58" @default.
• W2677486574 countsByYear W26774865742018 @default.
• W2677486574 countsByYear W26774865742019 @default.
• W2677486574 countsByYear W26774865742020 @default.
• W2677486574 countsByYear W26774865742021 @default.
• W2677486574 countsByYear W26774865742022 @default.
• W2677486574 countsByYear W26774865742023 @default.
• W2677486574 crossrefType "journal-article" @default.
• W2677486574 hasAuthorship W2677486574A5010362194 @default.
• W2677486574 hasAuthorship W2677486574A5017125420 @default.
• W2677486574 hasAuthorship W2677486574A5019415991 @default.
• W2677486574 hasAuthorship W2677486574A5021855910 @default.
• W2677486574 hasAuthorship W2677486574A5023498981 @default.
• W2677486574 hasAuthorship W2677486574A5043164290 @default.
• W2677486574 hasAuthorship W2677486574A5051471066 @default.
• W2677486574 hasAuthorship W2677486574A5058637743 @default.
• W2677486574 hasAuthorship W2677486574A5069143016 @default.
• W2677486574 hasAuthorship W2677486574A5075658046 @default.
• W2677486574 hasAuthorship W2677486574A5076620288 @default.
• W2677486574 hasBestOaLocation W26774865741 @default.
• W2677486574 hasConcept C17741926 @default.

• next