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• W2683152696 abstract "Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy." @default.
• W2683152696 created "2017-06-30" @default.
• W2683152696 creator A5036488360 @default.
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• W2683152696 date "2017-06-26" @default.
• W2683152696 modified "2023-10-18" @default.
• W2683152696 title "A DNA origami platform for quantifying protein copy number in super-resolution" @default.
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• W2683152696 doi "https://doi.org/10.1038/nmeth.4342" @default.
• W2683152696 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5534338" @default.
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• W2683152696 hasPublicationYear "2017" @default.
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