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- W268588734 abstract "Nucleotide sequences available in public database pr ovide a cost effective and valuable source for the development of molecular markers. In this study, the nucleotide se quence database available in National Centre for Bio technology Information (NCBI) is utilized to identify and develo p SSR markers in mungbean (Vigna radiata). A total of 803 genomic sequences, 829 EST sequences and 82 GSS sequences we re downloaded from NCBI. Eight hundred and forty two SS Rs from genomic sequences, 240 SSRs from EST sequences and 60 SSRs from GSS sequences were obtained using SSRIT tool. Primers pairs were successfully designed for 109 SSR motifs from genomic sequence, 110 SSR motifs from EST sequence and 25 SSR motifs from GSS sequences using Primer3 (http://frodo.wi.mit.edu) software. Fifteen SSR prim ers were finally characterized and validated in 24 mungbean and six urd bean accessions." @default.
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- W268588734 date "2013-01-01" @default.
- W268588734 modified "2023-10-12" @default.
- W268588734 title "Development of SSR markers in mung bean, Vigna radiata (L.) Wilczek using in silico methods." @default.
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