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- W2701397504 abstract "Ultraviolet radiation causes DNA damage, protein oxidation and generation of reactive oxygen species. This oxidative stress can produce skin damage, photoaging, inflammation or carcinogenesis. Some polyphenols, present in plant extracts, have shown antioxidant and photoprotective effects. This study evaluated the antioxidant effect of 9 Mediterranean plants extracts and their photoprotective effect against UVB radiation in human keratinocytes. The antioxidant activity of the extracts was evaluated in vitro with two different methods: ferric reducing-antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Photoprotection study was carried out determining the viability of HaCaT cells (keratinocytes) exposed to UVB through MTT assay. The cells were treated with 100-200 µg/mL of the different extracts. In addition, the antioxidant activity was determined in the photoprotection assay, using the ROS-sensitive dye 2’, 7’-dichlorofluorescein diacetate (DCF-DA). The extracts showed high antioxidant capacity and a photoprotective effect against UVB-induced ROS production in HaCaT cells. These results show that these Mediterranean extracts may be considered as a potential ingredient for photoprotection." @default.
- W2701397504 created "2017-06-30" @default.
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- W2701397504 date "2017-07-01" @default.
- W2701397504 modified "2023-09-27" @default.
- W2701397504 title "Antioxidant activity and photoprotection of Mediterranean plants extracts" @default.
- W2701397504 doi "https://doi.org/10.1016/j.freeradbiomed.2017.04.296" @default.
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