Matches in SemOpenAlex for { <https://semopenalex.org/work/W27086249> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W27086249 endingPage "72" @default.
- W27086249 startingPage "959" @default.
- W27086249 abstract "Antiserum to cobalamin was raised in rabbits by immunization with the monocarboxyl derivative of cyanocobalamin coupled to albumin. The antiserum was treated to remove transcobalamin II and transcobalamin I. The partially purified antibody bound free cyano[57Co]cobalamin, but not the vitamin precoupled to the transcobalamins. Cyano[57Co]cobalamin bound by the antiserum eluted from Sephadex G-200 as a single peak with a mol wt of 160,000 and was precipitated by goat anti-rabbit gamma globulin, indicating that the vitamin was bound to an IgG immunoglobulin. Unlabeled cyanocobalamin and hydroxocobalamin competitively inhibited the binding of cyano[57Co]cobalamin to this antibody. Neither adenosylcobalamin, in a similar concentration range, nor cyanocobinamide at a concentration 30-fold greater than the tracer cobalamin competed appreciably with the binding of cyano[57Co] cobalamin. The association constant for the interaction of the antibody with cyanocobalamin and cyanocobinamide was estimated to be 8.6 X 10(9) and 9.6 X 10(6) L/mol, respectively. The association constant for adenosylcobalamin, methylcobalamin, and hydroxocobalamin was indirectly determined, and values of 2.5 X 10(5), 1.7 X 10(9), and 2.3 X 10(9) L/mol, respectively, were obtained. Photolysis in the presence of potassium cyanide rendered each of the three cobalamins equivalent to cyanocobalamin in immunoreactivity. The mean concentration of cobalamin in normal human sera and cobalamin-deficient sera measured as cyanocobalamin by radioimmunoassay using this anticobalamin antibody was significantly lower than the concentration measured in the same extracts by competitive ligand-binding radioassays using intrinsic factor and transcobalamin I. These findings, although indirect, support the proposition that there may be factor(s) in normal and cobalamin-deficient sera that falsely elevate the concentration of true cobalamin if the radioassay uses R protein as the binder. However, the lower concentration of serum cobalamin measured by radioimmunoassay compared with the intrinsic factor radioassay also indicates that this purported factor(s) reacts to some extent with intrinsic factor but not with the cobalamin antibody." @default.
- W27086249 created "2016-06-24" @default.
- W27086249 creator A5056019879 @default.
- W27086249 creator A5062337224 @default.
- W27086249 creator A5083684292 @default.
- W27086249 creator A5084689319 @default.
- W27086249 date "1984-06-01" @default.
- W27086249 modified "2023-10-17" @default.
- W27086249 title "Measurement of cyanocobalamin in serum by a specific radioimmunoassay." @default.
- W27086249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6726060" @default.
- W27086249 hasPublicationYear "1984" @default.
- W27086249 type Work @default.
- W27086249 sameAs 27086249 @default.
- W27086249 citedByCount "4" @default.
- W27086249 countsByYear W270862492013 @default.
- W27086249 crossrefType "journal-article" @default.
- W27086249 hasAuthorship W27086249A5056019879 @default.
- W27086249 hasAuthorship W27086249A5062337224 @default.
- W27086249 hasAuthorship W27086249A5083684292 @default.
- W27086249 hasAuthorship W27086249A5084689319 @default.
- W27086249 hasConcept C159654299 @default.
- W27086249 hasConcept C163994747 @default.
- W27086249 hasConcept C176685330 @default.
- W27086249 hasConcept C181199279 @default.
- W27086249 hasConcept C185592680 @default.
- W27086249 hasConcept C203014093 @default.
- W27086249 hasConcept C2776619502 @default.
- W27086249 hasConcept C2777569460 @default.
- W27086249 hasConcept C2778979354 @default.
- W27086249 hasConcept C2779750884 @default.
- W27086249 hasConcept C2779761754 @default.
- W27086249 hasConcept C2780676250 @default.
- W27086249 hasConcept C2780829489 @default.
- W27086249 hasConcept C43617362 @default.
- W27086249 hasConcept C55493867 @default.
- W27086249 hasConcept C86803240 @default.
- W27086249 hasConcept C91523082 @default.
- W27086249 hasConceptScore W27086249C159654299 @default.
- W27086249 hasConceptScore W27086249C163994747 @default.
- W27086249 hasConceptScore W27086249C176685330 @default.
- W27086249 hasConceptScore W27086249C181199279 @default.
- W27086249 hasConceptScore W27086249C185592680 @default.
- W27086249 hasConceptScore W27086249C203014093 @default.
- W27086249 hasConceptScore W27086249C2776619502 @default.
- W27086249 hasConceptScore W27086249C2777569460 @default.
- W27086249 hasConceptScore W27086249C2778979354 @default.
- W27086249 hasConceptScore W27086249C2779750884 @default.
- W27086249 hasConceptScore W27086249C2779761754 @default.
- W27086249 hasConceptScore W27086249C2780676250 @default.
- W27086249 hasConceptScore W27086249C2780829489 @default.
- W27086249 hasConceptScore W27086249C43617362 @default.
- W27086249 hasConceptScore W27086249C55493867 @default.
- W27086249 hasConceptScore W27086249C86803240 @default.
- W27086249 hasConceptScore W27086249C91523082 @default.
- W27086249 hasIssue "6" @default.
- W27086249 hasLocation W270862491 @default.
- W27086249 hasOpenAccess W27086249 @default.
- W27086249 hasPrimaryLocation W270862491 @default.
- W27086249 hasRelatedWork W2009784004 @default.
- W27086249 hasRelatedWork W2016160434 @default.
- W27086249 hasRelatedWork W2039811446 @default.
- W27086249 hasRelatedWork W2043807045 @default.
- W27086249 hasRelatedWork W2075050566 @default.
- W27086249 hasRelatedWork W2076526297 @default.
- W27086249 hasRelatedWork W2151953480 @default.
- W27086249 hasRelatedWork W2338736715 @default.
- W27086249 hasRelatedWork W27086249 @default.
- W27086249 hasRelatedWork W2774118088 @default.
- W27086249 hasVolume "103" @default.
- W27086249 isParatext "false" @default.
- W27086249 isRetracted "false" @default.
- W27086249 magId "27086249" @default.
- W27086249 workType "article" @default.