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- W2711288014 abstract "Spheroids which cells aggregate and form have far greater functions than individual cells. In particular, hetero-spheroid composed of different cell species such as hepatocytes and endothelial cells express tissue specific function at a high level, which will realize more precise drag screening and biological research. In this study, we propose rapid formation of three-dimensional hetero-spheroid consisting of hepatocytes and endothelial cells using micro-rotation flow. Hepatocytes are covered by collagen gel layers, whose thickness are less than 200 μm, to increase adhesion strength between hepatocytes and endothelial cells. Gel-coated hepatocytes and endothelial cells are collected to form the hetero-spheroids in array by micro-rotational flow in two minutes. This array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations of less than 19 % by varying the cell density of the medium without altering the device geometry." @default.
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- W2711288014 date "2011-01-01" @default.
- W2711288014 modified "2023-10-16" @default.
- W2711288014 title "1A2-C01 Formation of three dimensional hetero-spheroid using micro-rotational flow(Nano/Micro Fluid System)" @default.
- W2711288014 doi "https://doi.org/10.1299/jsmermd.2011._1a2-c01_1" @default.
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