Matches in SemOpenAlex for { <https://semopenalex.org/work/W2717637612> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2717637612 endingPage "57" @default.
- W2717637612 startingPage "53" @default.
- W2717637612 abstract "Neutralizing antibodies (nAbs) play an important role in protective immunity against porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, the characterization of PRRSV nAb repertoires is rarely investigated. In this study, we developed a swine VDJ amplification method and selection criteria for the characterization of PRRSV-activated VDJ repertoires. According to clonal expansion theory, two separated aliquots of lymph nodes from pigs producing different PRRSV nAbs were utilized to determine the activated B-cell repertoires. Swine VDJ repertoires from a mock-infected pig and PRRSV-infected pigs secreting no detectable nAbs, only homologous nAbs, and broad nAbs were amplified by a single pair of primers that could detect all seven major VDJ genes. The amplicons were cloned and sequenced to generate 385 VDJ sequences. Sequence alignment showed that the diversification of VDJ genes was mainly due to the variation in complementarity determining regions (CDRs), especially CDR3. Based on selection criteria, shared and abundant sequences were identified in two separated aliquots from PRRSV-infected pigs but not from the mock-infected pig, suggesting they were secreted from PRRSV-activated B cells. Thus, the amplification and selection method provide a potential alternative for the characterization of swine VDJ repertoires. However, additional experiments are required to determine whether the shared and abundant VDJ lineages identified in this study are PRRSV-specific or distinct neutralizing-antibodies-associated." @default.
- W2717637612 created "2017-06-30" @default.
- W2717637612 creator A5010952641 @default.
- W2717637612 creator A5025864599 @default.
- W2717637612 creator A5075288916 @default.
- W2717637612 date "2017-07-01" @default.
- W2717637612 modified "2023-09-24" @default.
- W2717637612 title "Amplification and selection of PRRSV-activated VDJ repertoires in pigs secreting distinct neutralizing antiboidies" @default.
- W2717637612 cites W135539280 @default.
- W2717637612 cites W1526789785 @default.
- W2717637612 cites W1532823268 @default.
- W2717637612 cites W1579473839 @default.
- W2717637612 cites W1836840798 @default.
- W2717637612 cites W1968091806 @default.
- W2717637612 cites W1970534028 @default.
- W2717637612 cites W1971707621 @default.
- W2717637612 cites W1975698820 @default.
- W2717637612 cites W1991071535 @default.
- W2717637612 cites W1999423095 @default.
- W2717637612 cites W2009518556 @default.
- W2717637612 cites W2012121871 @default.
- W2717637612 cites W2014689076 @default.
- W2717637612 cites W2022221356 @default.
- W2717637612 cites W2025031008 @default.
- W2717637612 cites W2055179552 @default.
- W2717637612 cites W2057065295 @default.
- W2717637612 cites W2080084332 @default.
- W2717637612 cites W2104824773 @default.
- W2717637612 cites W2106908706 @default.
- W2717637612 cites W2121064478 @default.
- W2717637612 cites W2125518194 @default.
- W2717637612 cites W2134803208 @default.
- W2717637612 cites W2142467502 @default.
- W2717637612 cites W2152207030 @default.
- W2717637612 cites W2155855550 @default.
- W2717637612 cites W2170938141 @default.
- W2717637612 cites W2195363237 @default.
- W2717637612 cites W4235232179 @default.
- W2717637612 doi "https://doi.org/10.1016/j.vetimm.2017.06.005" @default.
- W2717637612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28669387" @default.
- W2717637612 hasPublicationYear "2017" @default.
- W2717637612 type Work @default.
- W2717637612 sameAs 2717637612 @default.
- W2717637612 citedByCount "2" @default.
- W2717637612 countsByYear W27176376122021 @default.
- W2717637612 countsByYear W27176376122022 @default.
- W2717637612 crossrefType "journal-article" @default.
- W2717637612 hasAuthorship W2717637612A5010952641 @default.
- W2717637612 hasAuthorship W2717637612A5025864599 @default.
- W2717637612 hasAuthorship W2717637612A5075288916 @default.
- W2717637612 hasConcept C104317684 @default.
- W2717637612 hasConcept C142724271 @default.
- W2717637612 hasConcept C159047783 @default.
- W2717637612 hasConcept C159654299 @default.
- W2717637612 hasConcept C2522874641 @default.
- W2717637612 hasConcept C2779134260 @default.
- W2717637612 hasConcept C2779656172 @default.
- W2717637612 hasConcept C2779819720 @default.
- W2717637612 hasConcept C3008058167 @default.
- W2717637612 hasConcept C524204448 @default.
- W2717637612 hasConcept C54355233 @default.
- W2717637612 hasConcept C71924100 @default.
- W2717637612 hasConcept C86803240 @default.
- W2717637612 hasConceptScore W2717637612C104317684 @default.
- W2717637612 hasConceptScore W2717637612C142724271 @default.
- W2717637612 hasConceptScore W2717637612C159047783 @default.
- W2717637612 hasConceptScore W2717637612C159654299 @default.
- W2717637612 hasConceptScore W2717637612C2522874641 @default.
- W2717637612 hasConceptScore W2717637612C2779134260 @default.
- W2717637612 hasConceptScore W2717637612C2779656172 @default.
- W2717637612 hasConceptScore W2717637612C2779819720 @default.
- W2717637612 hasConceptScore W2717637612C3008058167 @default.
- W2717637612 hasConceptScore W2717637612C524204448 @default.
- W2717637612 hasConceptScore W2717637612C54355233 @default.
- W2717637612 hasConceptScore W2717637612C71924100 @default.
- W2717637612 hasConceptScore W2717637612C86803240 @default.
- W2717637612 hasFunder F4320321001 @default.
- W2717637612 hasFunder F4320321543 @default.
- W2717637612 hasLocation W27176376121 @default.
- W2717637612 hasLocation W27176376122 @default.
- W2717637612 hasOpenAccess W2717637612 @default.
- W2717637612 hasPrimaryLocation W27176376121 @default.
- W2717637612 hasRelatedWork W1679554208 @default.
- W2717637612 hasRelatedWork W1968017175 @default.
- W2717637612 hasRelatedWork W1981211838 @default.
- W2717637612 hasRelatedWork W2009705525 @default.
- W2717637612 hasRelatedWork W2021279622 @default.
- W2717637612 hasRelatedWork W2040248008 @default.
- W2717637612 hasRelatedWork W2154632393 @default.
- W2717637612 hasRelatedWork W2213278224 @default.
- W2717637612 hasRelatedWork W2379951991 @default.
- W2717637612 hasRelatedWork W2417428323 @default.
- W2717637612 hasVolume "189" @default.
- W2717637612 isParatext "false" @default.
- W2717637612 isRetracted "false" @default.
- W2717637612 magId "2717637612" @default.
- W2717637612 workType "article" @default.