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- W27185518 abstract "This chapter describes experimental approaches by which the phagocytic activity of virtually tens of thousands of cells in a population of macrophages can be rapidly measured in vitro on a cell-by-cell basis using flow cytometry. Phagocytosis measured by flow cytometry can be quantitatively described in two ways—namely, (1) the percentage of cells with engulfed particles can be determined and (2) individual particulate burdens within each cell can be expressed as frequency distributions showing the frequency of phagocytic cells with a given number of internalized particles. Cell populations should be maintained in a complete medium that is buffered with an organic buffer and not with sodium bicarbonate. Macrophages are exquisitely sensitive to a pH exceeding 7.4. The cells are analyzed for narrow angle light scatter (cell size) and for fluorescence (phagocytized spheres) as they flow through a flow cell and intersect a laser beam of exciting light. Optical sensors measure light scattering and fluorescence on a cell-by-cell basis and the signals are stored in the list mode data format in a computer. The data is then reprocessed and displayed as single- or two-parameter cell-size and fluorescence frequency distribution histograms." @default.
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- W27185518 date "1986-01-01" @default.
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- W27185518 title "[4] In Vitro and in Vivo measurement of phagocytosis by flow cytometry" @default.
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- W27185518 doi "https://doi.org/10.1016/s0076-6879(86)32006-8" @default.
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