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- W272116100 abstract "C3d is the final split product of C3.The C3d cDNA was cloned from the liver of AA chickens through RT-PCR.C3d cDNA was proved to be the C3d gene of chicken by sequencing.Then,the gene was cloned into pET-32a(+),and transformed the recombinant plasmid into BL21(DE3)and induced with 1.0 mmol/L IPTG for gene expression.The expressed product was identified by SDS-PAGE and Western-blotting,it was approved that the recombinant protein was recombinant C3d.It was isolated and purified by the method of affinity chromatography.The protein conjugation to inactive NDV(Lasota strain)by glutaual was injected to the SPF chicken.monitored The dynamic changes of T lymphocyte proliferative responses in thymus and the kinetic changes of antibody of immunized chickens were by MTT method and HI method.It was proved that the recombinant C3d protein could enhance both the humoral and cellular immune response of the inactive NDV vaccine.This report constituted a solid foundation for further researches of chicken C3d." @default.
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- W272116100 date "2008-01-01" @default.
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- W272116100 title "Increased immunogenicity by conjugation of recombinant complement C3d to inactived NDV vaccine" @default.
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