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- W2726024069 abstract "Avian myogenesis is partly characterized by commitment of distinct myoblast cell lineages to the formation of specific muscle fiber types. Previous studies have identified the transcription factor EMX2 as a regulator of slow myosin heavy chain 2 (MyHC2) gene expression in fast/slow primary muscle fibers. We report here the interaction of EMX2 with the slow MyHC2 transcriptional regulatory region in fast/slow embryonic muscle fibers. Promoter activity and electromobility shift assays localized the site of interaction of EMX2 with the slow MyHC2 gene within a defined binding site located between 3336 and 3326 bp from the 3′ end of the cloned slow MyHC2 DNA containing the transcriptional regulatory region. Using clonally-derived myoblasts stably committed to the formation of fast/slow muscle fibers, we also report the effect of altered EMX2 gene expression on genome-wide gene expression within these myoblasts. Increased EMX2 gene expression in fast/slow myoblasts caused altered gene expression of 1185 genes, indicating that EMX2 plays a central role in the gene expression profile of embryonic myoblasts." @default.
- W2726024069 created "2017-07-14" @default.
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- W2726024069 date "2017-10-01" @default.
- W2726024069 modified "2023-10-13" @default.
- W2726024069 title "EMX2 activates slow myosin heavy chain 2 gene expression in embryonic muscle fibers" @default.
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- W2726024069 doi "https://doi.org/10.1016/j.mod.2017.06.006" @default.
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