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• W2727832831 abstract "The aim of this study was to evaluate the influence of different concentrations of water salinity on the apoptosis of germinal cells of the seminiferous tubules from Morada Nova lambs. Thirty two Morada Nova lambs with seven months were used. The animals were distributed into four treatments according to the levels of sodium chloride (NaCl) added to the water for them to drink, and consequently, the levels of total dissolved solids (TDS): 665 (control - treatment 1), 3.322 (treatment 2), 5.958 (treatment 3) or 8.614 (treatment 4) mg/L TDS. After 75 days, animals were killed and the testes were destined to histology and therefore to analysis of apoptosis in the germinal cells through TUNEL technique, using a kit for cell death detection (Roche Diagnostics Ltd., Indianapolis, USA). Sections of 5 μm were mounted on glass slides. Antigen retrieval was performed in sodium citrate buffer (Dinâmica, Sao Paulo, Brazil) for 10 min. Endogenous peroxidase activity was blocked by 3% H2O2 (Dinâmica) in methanol (Qeel, Sao Paulo, Brasil) for 10 min. The sections were incubated with TUNEL reaction mixture for 30 min. Then, the specimens were incubated with Converter-POD for 30 min. The DNA fragmentation was revealed by incubation of the tissues with diaminobenzidine (DAB; 0.05% DAB; Sigma, St. Louis, EUA) during 1 min. Finally, sections were counterstained with haematoxylin (Vetec, Sao Paulo, Brazil). For negative controls, slides were incubated with label solution (without terminal deoxynucleotidyl transferase enzyme) instead of TUNEL reaction mixture. The number of TUNEL positive cells was counted in randomly fields per treatment using Image-Pro Plus® software. The cells were considered TUNEL positive when they showed a brown staining. All data were submitted to Quisquare test (P<0.05). The results showed that there was a significant increase in the percentage of TUNEL positive cells in treatment 4 (9.33%), i.e. the treatment with higher proportion of TDS, when compared with the control (0.4%) and other treatments (1.23% e 0.8% for treatments 2 and 3, respectively). There was no staining in the negative control. In conclusion, 8.614 mg/mL NaCl added to the water increases apoptosis in early spermatogonia and spermatide of the seminiferous tubules from Morada Nova lambs." @default.
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• W2727832831 date "2014-03-11" @default.
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• W2727832831 title "Effect of water intake with different salinity levels on the apoptosis of germinal epithelium cells from Morada Nova lambs" @default.
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