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- W2727915066 abstract "Most gene clusters encoding multiprotein complexes of the bacterial cell envelope, such as conjugation and secretion systems, Type IV pili, and flagella, bear a gene encoding an enzyme with peptidoglycan hydrolase activity. These enzymes are usually glycoside hydrolases that cleave the glycan chains of the peptidoglycan. Their activities are spatially controlled to avoid cell lysis and to create localized rearrangement of the cell wall. This is assured by interaction with the structural subunits of the apparatus. Here we describe protocols to test the peptidoglycan hydrolase activity of these proteins in vitro and in solution." @default.
- W2727915066 created "2017-07-14" @default.
- W2727915066 creator A5018842385 @default.
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- W2727915066 date "2017-01-01" @default.
- W2727915066 modified "2023-10-14" @default.
- W2727915066 title "Measure of Peptidoglycan Hydrolase Activity" @default.
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- W2727915066 doi "https://doi.org/10.1007/978-1-4939-7033-9_12" @default.
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