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• W2728904428 startingPage "305" @default.
• W2728904428 abstract "Histamine (HA) is a biogenic amine that can accumulate to high concentration levels in food as a result of microbial activity and can cause toxic effects in consumers. In this work, a portable electrochemical immunosensor capable of detecting HA with high sensitivity and selectivity was developed. Prussian blue-chitosan-gold nanoparticle (PB-CS-AuNP) nanocomposite films with excellent biocompatibility were synthesized and characterized by scanning electron microscopy and energy dispersive X-ray analysis. The PB-CS-AuNP were coated onto a screen-printed electrode by one-step electrodeposition and used to conjugate the HA ovalbumin conjugate (HA-Ag). HA was determined by a competition between the coating HA-Ag and the HRP labeled HA antibody (HRP-HA-Ab). After careful optimization of assay conditions and Box-Behnken analysis, the developed immunosensor showed a linear range from 0.01 to 100 μg/mL for HA in fish samples. The average recoveries from spiked samples ranged from 97.25% to 105%. The biosensor also showed good specificity, reproducibility, and stability, indicating its potential application in monitoring HA in a simple and low cost manner." @default.
• W2728904428 created "2017-07-14" @default.
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• W2728904428 date "2017-12-01" @default.
• W2728904428 modified "2023-10-16" @default.
• W2728904428 title "Portable amperometric immunosensor for histamine detection using Prussian blue-chitosan-gold nanoparticle nanocomposite films" @default.
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• W2728904428 doi "https://doi.org/10.1016/j.bios.2017.07.014" @default.
• W2728904428 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5762249" @default.
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