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- W2728991310 abstract "Visualization of chromosomal loci location and dynamics is crucial for understanding many fundamental intra-nuclear processes such as DNA transcription, replication, and repair. Here, we will describe the development of fluorescence labeling methods for chromatin imaging, including traditional as well as emerging chromatin labeling techniques in both fixed and live cells.We will also discuss current issues and provide a perspective on future developments and applications of the chromatin labeling technology. A systematic literature search was performed using the PubMed. Studies published over the past 50 years were considered for review. More than 100 articles were cited in this review. Taking into account sensitivity, specificity, and spatiotemporal resolution, fluorescence labeling and imaging has been the most prevalent approach for chromatin visualization. Among all the fluorescent labeling tools, the adoption of genome editing tools, such as TALE and CRISPR, have great potential for the labeling and imaging of chromatin. Although a number of chromatin labeling techniques are available for both fixed and live cells, much more effort is still clearly required to develop fluorescence labeling methods capable of targeting arbitrary sequences non-intrusively to allow long-term, multiplexing, and high-throughput imaging of genomic loci and chromatin structures. The emerging technological advances will outline a next-generation effort toward the comprehensive delineation of chromatin at single-cell level with single-molecule resolution." @default.
- W2728991310 created "2017-07-14" @default.
- W2728991310 creator A5011403108 @default.
- W2728991310 creator A5025041192 @default.
- W2728991310 creator A5049567104 @default.
- W2728991310 creator A5061570552 @default.
- W2728991310 date "2017-06-27" @default.
- W2728991310 modified "2023-10-02" @default.
- W2728991310 title "Illuminating the structure and dynamics of chromatin by fluorescence labeling" @default.
- W2728991310 cites W1505917984 @default.
- W2728991310 cites W1531468991 @default.
- W2728991310 cites W1553227677 @default.
- W2728991310 cites W1567351510 @default.
- W2728991310 cites W1571241709 @default.
- W2728991310 cites W1782900133 @default.
- W2728991310 cites W1858357242 @default.
- W2728991310 cites W1889137377 @default.
- W2728991310 cites W1941076503 @default.
- W2728991310 cites W1962249777 @default.
- W2728991310 cites W1970831058 @default.
- W2728991310 cites W1972132227 @default.
- W2728991310 cites W1976647752 @default.
- W2728991310 cites W1977632393 @default.
- W2728991310 cites W1977944459 @default.
- W2728991310 cites W1978129709 @default.
- W2728991310 cites W1980430297 @default.
- W2728991310 cites W1983421390 @default.
- W2728991310 cites W1985039493 @default.
- W2728991310 cites W1986754361 @default.
- W2728991310 cites W1991149014 @default.
- W2728991310 cites W1999688054 @default.
- W2728991310 cites W2001793784 @default.
- W2728991310 cites W2003171404 @default.
- W2728991310 cites W2004448789 @default.
- W2728991310 cites W2006311225 @default.
- W2728991310 cites W2009993853 @default.
- W2728991310 cites W2017886758 @default.
- W2728991310 cites W2018349630 @default.
- W2728991310 cites W2021894549 @default.
- W2728991310 cites W2022658882 @default.
- W2728991310 cites W2023186182 @default.
- W2728991310 cites W2026180423 @default.
- W2728991310 cites W2026592619 @default.
- W2728991310 cites W2027315090 @default.
- W2728991310 cites W2028426939 @default.
- W2728991310 cites W2029742986 @default.
- W2728991310 cites W2030974598 @default.
- W2728991310 cites W2031468048 @default.
- W2728991310 cites W2032231011 @default.
- W2728991310 cites W2033132977 @default.
- W2728991310 cites W2033549300 @default.
- W2728991310 cites W2035420256 @default.
- W2728991310 cites W2043890495 @default.
- W2728991310 cites W2046493256 @default.
- W2728991310 cites W2047032063 @default.
- W2728991310 cites W2049046722 @default.
- W2728991310 cites W2051518111 @default.
- W2728991310 cites W2053270078 @default.
- W2728991310 cites W2056103949 @default.
- W2728991310 cites W2057793836 @default.
- W2728991310 cites W2060177007 @default.
- W2728991310 cites W2060485452 @default.
- W2728991310 cites W2063994725 @default.
- W2728991310 cites W2064038671 @default.
- W2728991310 cites W2064537118 @default.
- W2728991310 cites W2068427957 @default.
- W2728991310 cites W2069584416 @default.
- W2728991310 cites W2070021921 @default.
- W2728991310 cites W2070026305 @default.
- W2728991310 cites W2070614858 @default.
- W2728991310 cites W2070708756 @default.
- W2728991310 cites W2073378063 @default.
- W2728991310 cites W2075369115 @default.
- W2728991310 cites W2076438782 @default.
- W2728991310 cites W2080479776 @default.
- W2728991310 cites W2080551755 @default.
- W2728991310 cites W2085110456 @default.
- W2728991310 cites W2092499706 @default.
- W2728991310 cites W2095989610 @default.
- W2728991310 cites W2096261947 @default.
- W2728991310 cites W2096973725 @default.
- W2728991310 cites W2098017335 @default.
- W2728991310 cites W2103272211 @default.
- W2728991310 cites W2112124900 @default.
- W2728991310 cites W2112514675 @default.
- W2728991310 cites W2115003318 @default.
- W2728991310 cites W2117717914 @default.
- W2728991310 cites W2120699715 @default.
- W2728991310 cites W2127156350 @default.
- W2728991310 cites W2127540003 @default.
- W2728991310 cites W2128313472 @default.
- W2728991310 cites W2129788878 @default.
- W2728991310 cites W2131127199 @default.
- W2728991310 cites W2140099710 @default.
- W2728991310 cites W2142275143 @default.
- W2728991310 cites W2143539785 @default.
- W2728991310 cites W2143826692 @default.
- W2728991310 cites W2144252460 @default.