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- W2729122423 abstract "In many bacteria, membrane proteins account for around one-third of the proteome and can represent much more than half of the mass of a membrane. Classic techniques in cell biology can be applied to characterise bacterial membranes and their membrane protein constituents. Here we describe a protocol for the purification of outer and inner membranes from Escherichia coli. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane." @default.
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- W2729122423 date "2017-01-01" @default.
- W2729122423 modified "2023-10-16" @default.
- W2729122423 title "Defining Membrane Protein Localization by Isopycnic Density Gradients" @default.
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- W2729122423 doi "https://doi.org/10.1007/978-1-4939-7033-9_6" @default.
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