FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2729267984> ?p ?o ?g. }

• W2729267984 endingPage "e0180341" @default.
• W2729267984 startingPage "e0180341" @default.
• W2729267984 abstract "Biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. Biofilms are associated with chronic infections. Controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. Thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from it would be possible to develop inhibitors against bacterial colonization and potentiate antibiotherapy. To identify the key components and pathways that aid the opportunistic pathogen Pseudomonas aeruginosa in attachment we performed for the first time a proteomic analysis as early as after 20 minutes of incubation using glass wool fibers as a surface. We compared the protein contents of the attached and unattached bacteria. Using mass spectrometry, 3043 proteins were identified. Our results showed that, as of 20 minutes of incubation, using stringent quantification criteria 616 proteins presented a modification of their abundance in the attached cells compared to their unattached counterparts. The attached cells presented an overall reduced gene expression and characteristics of slow-growing cells. The over-accumulation of outer membrane proteins, periplasmic folding proteins and O-antigen chain length regulators was also observed, indicating a profound modification of the cell envelope. Consistently the sigma factor AlgU required for cell envelope homeostasis was highly over-accumulated in attached cells. In addition our data suggested a role of alarmone (p)ppGpp and polyphosphate during the early attachment phase. Furthermore, almost 150 proteins of unknown function were differentially accumulated in the attached cells. Our proteomic analysis revealed the existence of distinctive biological features in attached cells as early as 20 minutes of incubation. Analysis of some mutants demonstrated the interest of this proteomic approach in identifying genes involved in the early phase of adhesion to a surface." @default.
• W2729267984 created "2017-07-14" @default.
• W2729267984 creator A5017426163 @default.
• W2729267984 creator A5018748388 @default.
• W2729267984 creator A5025116732 @default.
• W2729267984 creator A5025418197 @default.
• W2729267984 creator A5037654559 @default.
• W2729267984 creator A5040012225 @default.
• W2729267984 creator A5053262964 @default.
• W2729267984 creator A5075490621 @default.
• W2729267984 creator A5089814079 @default.
• W2729267984 date "2017-07-05" @default.
• W2729267984 modified "2023-10-18" @default.
• W2729267984 title "Pseudomonas aeruginosa cells attached to a surface display a typical proteome early as 20 minutes of incubation" @default.
• W2729267984 cites W1502474321 @default.
• W2729267984 cites W1507688584 @default.
• W2729267984 cites W1593486525 @default.
• W2729267984 cites W171313903 @default.
• W2729267984 cites W1898572056 @default.
• W2729267984 cites W1915991060 @default.
• W2729267984 cites W1964344318 @default.
• W2729267984 cites W1966568212 @default.
• W2729267984 cites W1969537603 @default.
• W2729267984 cites W1973173116 @default.
• W2729267984 cites W1978413629 @default.
• W2729267984 cites W1983111267 @default.
• W2729267984 cites W1986748372 @default.
• W2729267984 cites W1989103500 @default.
• W2729267984 cites W1991484214 @default.
• W2729267984 cites W1993786223 @default.
• W2729267984 cites W1995924159 @default.
• W2729267984 cites W1996064131 @default.
• W2729267984 cites W1997900595 @default.
• W2729267984 cites W2000059703 @default.
• W2729267984 cites W2005988933 @default.
• W2729267984 cites W2009469402 @default.
• W2729267984 cites W2010466313 @default.
• W2729267984 cites W2015458663 @default.
• W2729267984 cites W2016158447 @default.
• W2729267984 cites W2018989138 @default.
• W2729267984 cites W2019844068 @default.
• W2729267984 cites W2023010129 @default.
• W2729267984 cites W2029738070 @default.
• W2729267984 cites W2033065712 @default.
• W2729267984 cites W2033825241 @default.
• W2729267984 cites W2036827681 @default.
• W2729267984 cites W2041854993 @default.
• W2729267984 cites W2044304587 @default.
• W2729267984 cites W2049110178 @default.
• W2729267984 cites W2052638688 @default.
• W2729267984 cites W2053943711 @default.
• W2729267984 cites W2055767311 @default.
• W2729267984 cites W2055792849 @default.
• W2729267984 cites W2061670564 @default.
• W2729267984 cites W2069549031 @default.
• W2729267984 cites W2070300719 @default.
• W2729267984 cites W2072180333 @default.
• W2729267984 cites W2075893586 @default.
• W2729267984 cites W2077193660 @default.
• W2729267984 cites W2084618758 @default.
• W2729267984 cites W2086876593 @default.
• W2729267984 cites W2095356769 @default.
• W2729267984 cites W2096173332 @default.
• W2729267984 cites W2097281897 @default.
• W2729267984 cites W2098992986 @default.
• W2729267984 cites W2099296814 @default.
• W2729267984 cites W2102072203 @default.
• W2729267984 cites W2103861833 @default.
• W2729267984 cites W2105783449 @default.
• W2729267984 cites W2106107840 @default.
• W2729267984 cites W2109038233 @default.
• W2729267984 cites W2111570722 @default.
• W2729267984 cites W2111693948 @default.
• W2729267984 cites W2113156590 @default.
• W2729267984 cites W2115175555 @default.
• W2729267984 cites W2117288172 @default.
• W2729267984 cites W2118342782 @default.
• W2729267984 cites W2118915737 @default.
• W2729267984 cites W2119037124 @default.
• W2729267984 cites W2122253611 @default.
• W2729267984 cites W2122937255 @default.
• W2729267984 cites W2124203749 @default.
• W2729267984 cites W2124219105 @default.
• W2729267984 cites W2125308047 @default.
• W2729267984 cites W2129723345 @default.
• W2729267984 cites W2130561341 @default.
• W2729267984 cites W2134440330 @default.
• W2729267984 cites W2135144856 @default.
• W2729267984 cites W2137055458 @default.
• W2729267984 cites W2140405224 @default.
• W2729267984 cites W2145613496 @default.
• W2729267984 cites W2148111565 @default.
• W2729267984 cites W2151077811 @default.
• W2729267984 cites W2154122150 @default.
• W2729267984 cites W2154136299 @default.
• W2729267984 cites W2155894000 @default.
• W2729267984 cites W2157240255 @default.
• W2729267984 cites W2158594038 @default.

• next