FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2729709219> ?p ?o ?g. }

• W2729709219 abstract "Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the effects of aerobic (DOT ≥30% air sat.), microaerobic (constant DOT = 3% air sat.) and oscillatory DOT (from 0 to 100% air sat.) conditions on pDNA production, quality and host performance were characterized. Microaerobic conditions had little effect on pDNA production, supercoiled fraction and sequence fidelity. By contrast, oscillatory DOT caused a 22% decrease in pDNA production compared with aerobic cultures. Although in aerobic cultures the pDNA supercoiled fraction was 98%, it decreased to 80% under heterogeneous DOT conditions. The different oxygen availabilities had no effect on the fidelity of the produced pDNA. The estimated metabolic fluxes indicated substantial differences at the level of the pentose phosphate pathway and TCA cycle under different conditions. Cyclic changes in fermentative pathway fluxes, as well as fast shifts in the fluxes through cytochromes, were also estimated. Model-based genetic modifications that can potentially improve the process performance are suggested. DOT heterogeneities strongly affected cell performance, pDNA production and topology. This should be considered when operating or scaling-up a bioreactor with deficient mixing. Constant microaerobic conditions affected the bacterial metabolism but not the amount or quality of pDNA. Therefore, pDNA production in microaerobic cultures may be an alternative for bioreactor operation at higher oxygen transfer rates." @default.
• W2729709219 created "2017-07-14" @default.
• W2729709219 creator A5016656069 @default.
• W2729709219 creator A5018123404 @default.
• W2729709219 creator A5019725092 @default.
• W2729709219 creator A5021815795 @default.
• W2729709219 creator A5070201382 @default.
• W2729709219 date "2017-07-04" @default.
• W2729709219 modified "2023-10-16" @default.
• W2729709219 title "Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli" @default.
• W2729709219 cites W1529785496 @default.
• W2729709219 cites W1970426717 @default.
• W2729709219 cites W1974470327 @default.
• W2729709219 cites W1976891441 @default.
• W2729709219 cites W1980740090 @default.
• W2729709219 cites W1980873743 @default.
• W2729709219 cites W1981906993 @default.
• W2729709219 cites W1988857358 @default.
• W2729709219 cites W1990592942 @default.
• W2729709219 cites W1996012051 @default.
• W2729709219 cites W2008841836 @default.
• W2729709219 cites W2015183433 @default.
• W2729709219 cites W2027068064 @default.
• W2729709219 cites W2027831635 @default.
• W2729709219 cites W2028362073 @default.
• W2729709219 cites W2041786205 @default.
• W2729709219 cites W2046246689 @default.
• W2729709219 cites W2048596643 @default.
• W2729709219 cites W2055666215 @default.
• W2729709219 cites W2069693934 @default.
• W2729709219 cites W2078355959 @default.
• W2729709219 cites W2079177608 @default.
• W2729709219 cites W2085011054 @default.
• W2729709219 cites W2097464440 @default.
• W2729709219 cites W2110572289 @default.
• W2729709219 cites W2113674809 @default.
• W2729709219 cites W2116137883 @default.
• W2729709219 cites W2124624490 @default.
• W2729709219 cites W2138266455 @default.
• W2729709219 cites W2139128100 @default.
• W2729709219 cites W2140305974 @default.
• W2729709219 cites W2171493278 @default.
• W2729709219 cites W2297386142 @default.
• W2729709219 cites W2310281749 @default.
• W2729709219 cites W2340682616 @default.
• W2729709219 cites W2398455741 @default.
• W2729709219 doi "https://doi.org/10.1186/s12896-017-0378-x" @default.
• W2729709219 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5496438" @default.
• W2729709219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28676110" @default.
• W2729709219 hasPublicationYear "2017" @default.
• W2729709219 type Work @default.
• W2729709219 sameAs 2729709219 @default.
• W2729709219 citedByCount "10" @default.
• W2729709219 countsByYear W27297092192017 @default.
• W2729709219 countsByYear W27297092192018 @default.
• W2729709219 countsByYear W27297092192019 @default.
• W2729709219 countsByYear W27297092192021 @default.
• W2729709219 crossrefType "journal-article" @default.
• W2729709219 hasAuthorship W2729709219A5016656069 @default.
• W2729709219 hasAuthorship W2729709219A5018123404 @default.
• W2729709219 hasAuthorship W2729709219A5019725092 @default.
• W2729709219 hasAuthorship W2729709219A5021815795 @default.
• W2729709219 hasAuthorship W2729709219A5070201382 @default.
• W2729709219 hasBestOaLocation W27297092191 @default.
• W2729709219 hasConcept C100544194 @default.
• W2729709219 hasConcept C104317684 @default.
• W2729709219 hasConcept C12554922 @default.
• W2729709219 hasConcept C168170006 @default.
• W2729709219 hasConcept C178790620 @default.
• W2729709219 hasConcept C185592680 @default.
• W2729709219 hasConcept C22744801 @default.
• W2729709219 hasConcept C2993207723 @default.
• W2729709219 hasConcept C540031477 @default.
• W2729709219 hasConcept C547475151 @default.
• W2729709219 hasConcept C552990157 @default.
• W2729709219 hasConcept C55493867 @default.
• W2729709219 hasConcept C59822182 @default.
• W2729709219 hasConcept C86803240 @default.
• W2729709219 hasConceptScore W2729709219C100544194 @default.
• W2729709219 hasConceptScore W2729709219C104317684 @default.
• W2729709219 hasConceptScore W2729709219C12554922 @default.
• W2729709219 hasConceptScore W2729709219C168170006 @default.
• W2729709219 hasConceptScore W2729709219C178790620 @default.
• W2729709219 hasConceptScore W2729709219C185592680 @default.
• W2729709219 hasConceptScore W2729709219C22744801 @default.
• W2729709219 hasConceptScore W2729709219C2993207723 @default.
• W2729709219 hasConceptScore W2729709219C540031477 @default.
• W2729709219 hasConceptScore W2729709219C547475151 @default.
• W2729709219 hasConceptScore W2729709219C552990157 @default.
• W2729709219 hasConceptScore W2729709219C55493867 @default.
• W2729709219 hasConceptScore W2729709219C59822182 @default.
• W2729709219 hasConceptScore W2729709219C86803240 @default.
• W2729709219 hasFunder F4320321739 @default.
• W2729709219 hasIssue "1" @default.
• W2729709219 hasLocation W27297092191 @default.
• W2729709219 hasLocation W27297092192 @default.
• W2729709219 hasLocation W27297092193 @default.
• W2729709219 hasLocation W27297092194 @default.
• W2729709219 hasOpenAccess W2729709219 @default.
• W2729709219 hasPrimaryLocation W27297092191 @default.

• next