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- W2730090876 abstract "This protocol describes the analysis, specifically the identification, of blood plasma lipids. Plasma lipids are extracted using methyl tert-butyl ether (MTBE), methanol, and water followed by separation and data acquisition of isolated lipids using reversed-phase liquid chromatography coupled to quadrupole/time-of-flight mass spectrometry (RPLC–QTOFMS) operated in MS/MS mode. For lipid identification, acquired MS/MS spectra are converted to the mascot generic format (MGF) followed by library search using the in-silico MS/MS library LipidBlast. Using this approach, lipid classes, carbon-chain lengths, and degree of unsaturation of fatty-acid components are annotated." @default.
- W2730090876 created "2017-07-14" @default.
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- W2730090876 date "2017-01-01" @default.
- W2730090876 modified "2023-10-16" @default.
- W2730090876 title "LC–MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library" @default.
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- W2730090876 doi "https://doi.org/10.1007/978-1-4939-6996-8_14" @default.
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