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- W2730775504 abstract "Since the first microbial genome has been finished in the year 1995 more than 800 sequenced microorganisms are publicly available and many more are on their way. Bioinformatic analysis of the sequence information is required to process this huge amount of data and to generate sound hypotheses in terms of physiology and ecology of an organism as well as to efficiently screen for candidate genes for biotechnological relevant enzymes.However, high-throughput lab-methods are more than ever required for the subsequent verification of initial bioinformatic findings and hypotheses. Post-genomic approaches like DNA microarrays have revolutionised molecular biology by enabling a new dimension of parallelism in expression profiling. Eight years ago, the Max Planck Institute for Marine Microbiology has initiated a genomics project for the complete genome sequencing and analysis of environmental relevant marine bacteria. Rhodopirellula baltica was selected as a marine representative of the globally distributed and environmentally important bacterial phylum Planctomycetales. It is a model organism for aerobic carbohydrate degradation in marine systems, where polysaccharides represent the dominant components of biomass. The complete genome sequence of R. baltica has been determined and functional annotation was performed. However, pure in silico predictions left about 55% of the genes without functional assignment. The availability of the whole genome sequence has already stimulated a set of follow-up studies ranging from proteomics to protein crystallization as well as biotechnological applications. This work presents the establishment of, and the results gained with, the first whole genome microarray for a Planctomycete. It shows that massive expression profiling under defined environmental conditions can take the challenge to bridge the gap between classical studies on the cell biology and physiology of Planctomycetes and their active genetic repertoire." @default.
- W2730775504 created "2017-07-14" @default.
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- W2730775504 date "2009-06-22" @default.
- W2730775504 modified "2023-09-27" @default.
- W2730775504 title "Ecological aspects of the marine planctomycete Rhodopirellula baltica SH1T : a whole genome array approach" @default.
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