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• W2731678056 abstract "HomePlant DiseaseVol. 101, No. 10First Report of Neopestalotiopsis saprophytica Causing Leaf Spot of Oil Palm (Elaeis guineensis) in Malaysia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Neopestalotiopsis saprophytica Causing Leaf Spot of Oil Palm (Elaeis guineensis) in MalaysiaS. I. Ismail, D. Zulperi, S. Norddin, and S. Ahmad-HamdaniS. I. Ismail†Corresponding author. E-mail: E-mail Address: izera@upm.edu.mySearch for more papers by this author, D. ZulperiSearch for more papers by this author, S. NorddinSearch for more papers by this author, and S. Ahmad-HamdaniSearch for more papers by this authorAffiliationsAuthors and Affiliations S. I. Ismail † D. Zulperi S. Norddin , Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia S. Ahmad-Hamdani , Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Published Online:15 Aug 2017https://doi.org/10.1094/PDIS-02-17-0271-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Oil palm (Elaeis guineensis Jacq.) is an economically important plantation crop cultivated in Malaysia. In April to May 2016, leaf spot symptoms were observed on leaves of 5-year-old oil palm, Tenera variety, at the oil palm plantation located at UPM, Serdang, Selangor, Malaysia. Disease incidence reached 60%, leading to poor growth. Initially, small (average 3 mm in diameter), round, yellow-brown spots appeared on leaves and later the spots enlarged to form necrotic blotches. Five symptomatic leaves were collected from five diseased oil palms. To isolate the pathogen, fragments (approximately 5 × 5 mm) were excised from the margin of the diseased leaf tissues, surface sterilized with 0.525% NaOCl for 1 min, rinsed two times with sterile distilled water, plated on potato dextrose agar (PDA), and incubated at 25°C. Colonies were white and cottony, aerial mycelia on surface, and concentric black conidiomata appeared on the plate after 7 days. Single spore isolation technique was used to obtain pure cultures, and a representative isolate SII was characterized further. Forty conidia were measured, averaging 26 µm long × 6.0 µm wide. Conidia were fusiform, straight or slightly curved, 5-celled, and constituted of three versicolorous median cells and two terminal cells. The two upper median cells were darker than the second cell from base. Conidia had two to three apical appendages and one basal appendage. Based on the colony and conidia characters, the fungal isolate was initially identified as Neopestalotiopsis sp. (Maharachchikumbura et al. 2014). To confirm the identity of the fungus, the internal transcribed spacer (ITS) rDNA region of isolate SII was amplified by PCR using ITS5/ITS4 primers (White et al. 1990). The ITS sequence was deposited in GenBank (KY606286). A BLASTn search (NCBI database) showed that isolate SII had 100% homology with Neopestalotiopsis saprophytica reference strain CBS 115452 (KM199345). To confirm pathogenicity, a 10-μl conidia suspension (105 spores/ml) of 7-day-old culture of N. saprophytica was used to inoculate each of five detached healthy leaves of oil palm (7 cm long) placed in Petri dishes containing moist filter paper and maintained in a moist chamber. For control treatment, five additional detached leaves were inoculated with sterilized distilled water. Five days after inoculation, leaf spot symptoms developed on the inoculated leaves, while controls remained asymptomatic. The symptoms were similar to those observed on diseased leaves of oil palm in the plantation. Neopestalotiopsis saprophytica was reisolated from the diseased leaves, which showed identical morphological characteristics to the initial isolates, confirming Koch’s postulates. Alignments were made using MAFFT v.7.0 (Katoh and Standley 2013) and a maximum likelihood phylogram was generated by MEGA v.6.0 (Tamura et al. 2013). Isolate SII clustered together with N. saprophytica reference strain CBS 115452 and MFLUCC 12-0282 (98% bootstrap). Neopestalotiopsis saprophytica has been reported causing fruit rot of Litsea rotundifolia and leaf spots of Magnolia sp. in China (Maharachchikumbura et al. 2014). To our knowledge, this is the first report of N. saprophytica causing leaf spot disease on oil palms (E. guineensis) in Malaysia.References:Katoh, K., and Standley, D. M. 2013. Mol. Biol. Evol. 30:772. https://doi.org/10.1093/molbev/mst010 Crossref, ISI, Google ScholarMaharachchikumbura, S. S. N., et al. 2014. Stud. Mycol. 79:121. https://doi.org/10.1016/j.simyco.2014.09.005 Crossref, ISI, Google ScholarTamura, K., et al. 2013. Mol. Biol. Evol. 30:2725. https://doi.org/10.1093/molbev/mst197 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego. Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 10 October 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Sep 2017Published: 15 Aug 2017First Look: 28 Jun 2017Accepted: 21 Jun 2017 Page: 1821 Information© 2017 The American Phytopathological SocietyCited byTaxonomic Advances from Fungal Flora Associated with Ferns and Fern-like Hosts in Northern Thailand3 February 2023 | Plants, Vol. 12, No. 3Isolation, identification and pathogenicity of fungi associated with leaf blotches in Tenera x Tenera (TxT) variety of oil palm in Malaysia22 October 2021 | Journal of Plant Pathology, Vol. 104, No. 1Identification, pathogenicity and effects of plant extracts on Neopestalotiopsis and Pseudopestalotiopsis causing fruit diseases19 November 2021 | Scientific Reports, Vol. 11, No. 1First Report of Leaf Spot on Paphiopedilum micranthum Caused by Neopestalotiopsis saprophytica in ChinaQian Qin, Zhixiang Lu, Zuzheng Lu, Liqiong Ding, Zhaojin Chi, and Bing Shan30 July 2020 | Plant Disease, Vol. 104, No. 10Strains of Neopestalotiopsis sp. are in vitro antagonists of Cryphonectria parasiticaBiological Control, Vol. 143" @default.
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• W2731678056 title "First Report of <i>Neopestalotiopsis saprophytica</i> Causing Leaf Spot of Oil Palm (<i>Elaeis guineensis</i>) in Malaysia" @default.
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