Matches in SemOpenAlex for { <https://semopenalex.org/work/W273175344> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W273175344 abstract "High enolase activity, as measured by the conversion of 2-phosphoglycerate to phosphoenolphyruvate, was found in the cytoplasm of Pyrococcus (an anaerobic, hyperthermophilic archaeon that grows optimally at 100{degree}C). In this organism, the enzyme probably functions in a sugar fermentation pathway. The enzyme was purified to homogeneity. It had a temperature optimum of >90 {degree}C, and a pH optimum of 8.1. The enzyme was extremely thermostable with a half time for inactivation at 100{degree}C of 40 min. In contrast, an enolase from yeast was inactivated in 1 min at 88{degree}C. Both the P. furiosus and yeast enzymes required a metal ion for activity, but whereas the yeast enzyme has an absolute requirement for Mg{sup ++} the P. furiosus enolase was equally active in the presence of Mn{sup ++}. Both enzymes were competitively inhibited by citrate. P. furiosus enolase, as for mesophilic enolases, probably has a homodimeric structure with subunit M{sub r} greater than 45,000. A highly conserved sequence of eight amino acids in the N-terminal region was found in enolases from P. furiosus and a wide range of other organisms including bacteria, yeast, birds, and mammals." @default.
- W273175344 created "2016-06-24" @default.
- W273175344 creator A5000865211 @default.
- W273175344 creator A5009375401 @default.
- W273175344 creator A5013370958 @default.
- W273175344 creator A5019908814 @default.
- W273175344 creator A5064221873 @default.
- W273175344 creator A5064639578 @default.
- W273175344 creator A5086644908 @default.
- W273175344 date "1993-12-31" @default.
- W273175344 modified "2023-09-28" @default.
- W273175344 title "Characterization of the glycolytic enzyme enolase which is abundant in the hyperthermophilic archaeon, Pyrococcus furiosus" @default.
- W273175344 doi "https://doi.org/10.2172/10124321" @default.
- W273175344 hasPublicationYear "1993" @default.
- W273175344 type Work @default.
- W273175344 sameAs 273175344 @default.
- W273175344 citedByCount "0" @default.
- W273175344 crossrefType "report" @default.
- W273175344 hasAuthorship W273175344A5000865211 @default.
- W273175344 hasAuthorship W273175344A5009375401 @default.
- W273175344 hasAuthorship W273175344A5013370958 @default.
- W273175344 hasAuthorship W273175344A5019908814 @default.
- W273175344 hasAuthorship W273175344A5064221873 @default.
- W273175344 hasAuthorship W273175344A5064639578 @default.
- W273175344 hasAuthorship W273175344A5086644908 @default.
- W273175344 hasConcept C104317684 @default.
- W273175344 hasConcept C181199279 @default.
- W273175344 hasConcept C185592680 @default.
- W273175344 hasConcept C188618488 @default.
- W273175344 hasConcept C203014093 @default.
- W273175344 hasConcept C204232928 @default.
- W273175344 hasConcept C2776943354 @default.
- W273175344 hasConcept C2779222958 @default.
- W273175344 hasConcept C2781216036 @default.
- W273175344 hasConcept C27911776 @default.
- W273175344 hasConcept C28165981 @default.
- W273175344 hasConcept C550995028 @default.
- W273175344 hasConcept C55428944 @default.
- W273175344 hasConcept C55493867 @default.
- W273175344 hasConcept C86803240 @default.
- W273175344 hasConceptScore W273175344C104317684 @default.
- W273175344 hasConceptScore W273175344C181199279 @default.
- W273175344 hasConceptScore W273175344C185592680 @default.
- W273175344 hasConceptScore W273175344C188618488 @default.
- W273175344 hasConceptScore W273175344C203014093 @default.
- W273175344 hasConceptScore W273175344C204232928 @default.
- W273175344 hasConceptScore W273175344C2776943354 @default.
- W273175344 hasConceptScore W273175344C2779222958 @default.
- W273175344 hasConceptScore W273175344C2781216036 @default.
- W273175344 hasConceptScore W273175344C27911776 @default.
- W273175344 hasConceptScore W273175344C28165981 @default.
- W273175344 hasConceptScore W273175344C550995028 @default.
- W273175344 hasConceptScore W273175344C55428944 @default.
- W273175344 hasConceptScore W273175344C55493867 @default.
- W273175344 hasConceptScore W273175344C86803240 @default.
- W273175344 hasLocation W2731753441 @default.
- W273175344 hasLocation W2731753442 @default.
- W273175344 hasOpenAccess W273175344 @default.
- W273175344 hasPrimaryLocation W2731753441 @default.
- W273175344 hasRelatedWork W1544679009 @default.
- W273175344 hasRelatedWork W1550664490 @default.
- W273175344 hasRelatedWork W1974145329 @default.
- W273175344 hasRelatedWork W1985428254 @default.
- W273175344 hasRelatedWork W1998041471 @default.
- W273175344 hasRelatedWork W2028779838 @default.
- W273175344 hasRelatedWork W2060864118 @default.
- W273175344 hasRelatedWork W2142729092 @default.
- W273175344 hasRelatedWork W2158543301 @default.
- W273175344 hasRelatedWork W273175344 @default.
- W273175344 isParatext "false" @default.
- W273175344 isRetracted "false" @default.
- W273175344 magId "273175344" @default.
- W273175344 workType "report" @default.