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- W2732081483 abstract "Maternal RhD alloimmunization is an inflammatory response against protein antigens in fetal red blood cells (RBC). However, not all women become alloimmunized when exposed to RhD+ fetal RBC. Thus, this study aimed to evaluate levels of inflammatory chemokines in RhD- pregnant women with erythrocyte alloimmunization. CXCL8, CXCL9, CCL5, and CXCL10 levels were determined from cell culture supernatants by flow cytometry in 46 (30 non-alloimmunized RhD− and 16 previously alloimmunized RhD−) pregnant women. CXCL8 levels were significantly higher (P<0.004) and CXCL9 (P<0.008) and CXCL10 (P<0.003) levels were significantly lower in alloimmunized pregnant women. No significant difference in CCL5 levels was detected between the groups. Fetal RHD genotyping was performed in the alloimmunized RhD− group by real-time PCR. Anti-D alloantibody was detected in 10 mothers and anti-D and -C in six mothers. Twelve fetuses were RHD positive and four were RHD negative. Further studies of serum chemokines and placenta tissue could provide a better understanding of the cells involved in the pathogenesis of maternal erythrocyte alloimmunization. Key words: chemokines, pregnancy, inflammation, flow cytometry, Rh(o) antigen, genotyping techniques, hemolytic disease of the fetus and newborn." @default.
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- W2732081483 date "2017-07-03" @default.
- W2732081483 modified "2023-10-18" @default.
- W2732081483 title "High Levels of CXCL8 and Low Levels of CXCL9 and CXCL10 in Women with Maternal RhD Alloimmunization" @default.
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- W2732081483 doi "https://doi.org/10.3389/fimmu.2017.00700" @default.
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