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• W2732971076 abstract "This study examines the impacts of the urokinase-type plasminogen activator (uPA) on the in vitro maturation (IVM) of bovine oocytes. Cumulus–oocyte complexes in IVM medium were treated with uPA, amiloride (an uPA inhibitor), dimethyl sulfoxide (DMSO) or left untreated (control group). After 24 h of IVM, oocytes were recovered for testing or were in vitro fertilized and cultured to the blastocyst stage. The factors examined in all groups were: (i) oocyte nuclear maturation (Hoëscht staining); (ii) oocyte cytoplasmic maturation (cortical granules, CGs, distribution assessed by LCA-FITC); (iii) oocyte and cumulus cell (CC) gene expression (RT-qPCR); and (iv) embryo development (cleavage rate and blastocyst yield). Oocytes subjected to uPA treatment showed rates of nuclear maturation and CG distribution patterns similar to controls ( P > 0.05), whereas lower rates of oocyte maturation were recorded in the amiloride group ( P < 0.05). Both in oocytes and CC, treatment with uPA did not affect the transcription of genes related to apoptosis, cell junctions, cell cycle or serpin protease inhibitors. In contrast, amiloride altered the expression of genes associated with cell junctions, cell cycle, oxidative stress and CC serpins. No differences were observed between the control and uPA group in cleavage rate or in blastocyst yield recorded on Days 7, 8 or 9 post-insemination. However, amiloride led to drastically reduced cleavage rate (28.5% vs 83.2%) and Day 9 embryo production (6.0% vs 21.0%) over the rates recorded for DMSO. These results indicate that the proteolytic activity of uPA is needed for successful oocyte maturation in bovine." @default.
• W2732971076 created "2017-07-14" @default.
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• W2732971076 date "2017-09-01" @default.
• W2732971076 modified "2023-10-07" @default.
• W2732971076 title "Effect of urokinase type plasminogen activator on in vitro bovine oocyte maturation" @default.
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• W2732971076 cites W1734003606 @default.
• W2732971076 cites W1824637123 @default.
• W2732971076 cites W1869809348 @default.
• W2732971076 cites W1968619433 @default.
• W2732971076 cites W1974738966 @default.
• W2732971076 cites W1977873050 @default.
• W2732971076 cites W1980225571 @default.
• W2732971076 cites W1983483832 @default.
• W2732971076 cites W1985934519 @default.
• W2732971076 cites W1987286190 @default.
• W2732971076 cites W1991600608 @default.
• W2732971076 cites W1994067484 @default.
• W2732971076 cites W1999337849 @default.
• W2732971076 cites W2000469450 @default.
• W2732971076 cites W2012080977 @default.
• W2732971076 cites W2019893685 @default.
• W2732971076 cites W2021238642 @default.
• W2732971076 cites W2026614921 @default.
• W2732971076 cites W2027079212 @default.
• W2732971076 cites W2028689338 @default.
• W2732971076 cites W2032300482 @default.
• W2732971076 cites W2033665165 @default.
• W2732971076 cites W2038194914 @default.
• W2732971076 cites W2040082269 @default.
• W2732971076 cites W2041412012 @default.
• W2732971076 cites W2041640014 @default.
• W2732971076 cites W2042114928 @default.
• W2732971076 cites W2047416689 @default.
• W2732971076 cites W2048854988 @default.
• W2732971076 cites W2051979951 @default.
• W2732971076 cites W2060916027 @default.
• W2732971076 cites W2061417741 @default.
• W2732971076 cites W2062564391 @default.
• W2732971076 cites W2064911312 @default.
• W2732971076 cites W2066823528 @default.
• W2732971076 cites W2069215557 @default.
• W2732971076 cites W2075619575 @default.
• W2732971076 cites W2090342731 @default.
• W2732971076 cites W2090418625 @default.
• W2732971076 cites W2092717733 @default.
• W2732971076 cites W2095847870 @default.
• W2732971076 cites W2100814573 @default.
• W2732971076 cites W2100864709 @default.
• W2732971076 cites W2101602571 @default.
• W2732971076 cites W2104484443 @default.
• W2732971076 cites W2107277218 @default.
• W2732971076 cites W2107848687 @default.
• W2732971076 cites W2114570899 @default.
• W2732971076 cites W2115286110 @default.
• W2732971076 cites W2116273877 @default.
• W2732971076 cites W2119759674 @default.
• W2732971076 cites W2124905042 @default.
• W2732971076 cites W2127332827 @default.
• W2732971076 cites W2138055111 @default.
• W2732971076 cites W2145620343 @default.
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• W2732971076 cites W2153323265 @default.
• W2732971076 cites W2155710513 @default.
• W2732971076 cites W2158481547 @default.
• W2732971076 cites W2160251030 @default.
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• W2732971076 cites W2184083645 @default.
• W2732971076 cites W2264862919 @default.
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• W2732971076 doi "https://doi.org/10.1530/rep-17-0204" @default.
• W2732971076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28667127" @default.
• W2732971076 hasPublicationYear "2017" @default.
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