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• W2733403350 abstract "Aberrant activation of the transcription factor NF- κ B, as well as uncontrolled inflammation, has been linked to autoimmune diseases, development and progression of cancer, and neurological disorders like Alzheimer’s disease. Reporter cell lines are a valuable state-of-the art tool for comparative analysis of in vitro drug screening. However, a reporter cell line for the investigation of NF- κ B-driven neuroinflammation has not been available. Thus, we developed a stable neural NF- κ B-reporter cell line to assess the potency of proinflammatory molecules and peptides, as well as anti-inflammatory pharmaceuticals. We used lentivirus to transduce the glioma cell line U251-MG with a tandem NF- κ B reporter construct containing GFP and firefly luciferase allowing an assessment of NF- κ B activity via fluorescence microscopy, flow cytometry, and luminometry. We observed a robust activation of NF- κ B after exposure of the reporter cell line to tumour necrosis factor alpha (TNF α ) and amyloid- β peptide [1-42] as well as to LPS derived from Salmonella minnesota and Escherichia coli . Finally, we demonstrate that the U251-NF- κ B-GFP-Luc reporter cells can be used for assessing the anti-inflammatory potential of pharmaceutical compounds using Bay11-7082 and IMD0354. In summary, our newly generated cell line is a robust and cost-efficient tool to study pro- and anti-inflammatory potential of drugs and biologics in neural cells." @default.
• W2733403350 created "2017-07-14" @default.
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• W2733403350 date "2017-01-01" @default.
• W2733403350 modified "2023-10-14" @default.
• W2733403350 title "Development and Characterisation of a Novel NF-<i>κ</i>B Reporter Cell Line for Investigation of Neuroinflammation" @default.
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• W2733403350 doi "https://doi.org/10.1155/2017/6209865" @default.
• W2733403350 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5534271" @default.
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• W2733403350 hasPublicationYear "2017" @default.
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