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• W2733744796 abstract "We report the synthesis of atom-specifically 13C-modified building blocks that can be incorporated into DNA via solid phase synthesis to facilitate investigations on structural and dynamic features via NMR spectroscopy. In detail, 6-13C-modified pyrimidine and 8-13C purine DNA phosphoramidites were synthesized and incorporated into a polypurine tract DNA/RNA hybrid duplex to showcase the facile resonance assignment using site-specific labeling. We also addressed micro- to millisecond dynamics in the mini-cTAR DNA. This DNA is involved in the HIV replication cycle and our data points toward an exchange process in the lower stem of the hairpin that is up-regulated in the presence of the HIV-1 nucleocapsid protein 7. As another example, we picked a G-quadruplex that was earlier shown to exist in two folds. Using site-specific 8-13C-2'deoxyguanosine labeling we were able to verify the slow exchange between the two forms on the chemical shift time scale. In a real-time NMR experiment the re-equilibration of the fold distribution after a T-jump could be monitored yielding a rate of 0.012 min-1. Finally, we used 13C-ZZ-exchange spectroscopy to characterize the kinetics between two stacked X-conformers of a Holliday junction mimic. At 25°C, the refolding process was found to occur at a forward rate constant of 3.1 s-1 and with a backward rate constant of 10.6 s-1." @default.
• W2733744796 created "2017-07-14" @default.
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• W2733744796 date "2017-07-07" @default.
• W2733744796 modified "2023-10-14" @default.
• W2733744796 title "Synthesis and incorporation of 13C-labeled DNA building blocks to probe structural dynamics of DNA by NMR" @default.
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• W2733744796 cites W1966428896 @default.
• W2733744796 cites W1970802736 @default.
• W2733744796 cites W1972094845 @default.
• W2733744796 cites W1975277407 @default.
• W2733744796 cites W1977998433 @default.
• W2733744796 cites W1980513386 @default.
• W2733744796 cites W1982119742 @default.
• W2733744796 cites W1986563937 @default.
• W2733744796 cites W1993034742 @default.
• W2733744796 cites W1994261851 @default.
• W2733744796 cites W1995626688 @default.
• W2733744796 cites W1997822412 @default.
• W2733744796 cites W2000863706 @default.
• W2733744796 cites W2001962170 @default.
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• W2733744796 cites W2016959358 @default.
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• W2733744796 cites W2036319267 @default.
• W2733744796 cites W2036601571 @default.
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• W2733744796 cites W2045176942 @default.
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• W2733744796 doi "https://doi.org/10.1093/nar/gkx592" @default.
• W2733744796 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5587810" @default.
• W2733744796 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28911104" @default.
• W2733744796 hasPublicationYear "2017" @default.
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