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- W2733985235 abstract "The objectives of this study were to characterize follicle population, quality and number of oocytes recovered and in vitro embryo production in pre-pubertal Nelore heifers previously exposed to progesterone (P4). The experimental design included 42 Nelore females, distributed into 4 experimental groups, as follows: PP-P4-24d- pre-pubertal heifers undergoing (OPU) previously exposed to P4 (CIDR®, Zoetis) for 24 days n=11); PP-P4-7d– pre-pubertal heifers undergoing OPU previously exposed to P4 for 7 days (n=11); PP – pre-pubertal heifers undergoing OPU without previous P4 priming (Negative Control, n=10); and VC – mature cycling cows undergoing OPU previously exposed to P4 for 7 days ( n=10). On D0, heifers in PP-P4-24d received a CIDR®, which was removed on D7 of the protocol. These same animals received another P4 device on Day 14. Animals in groups PP-P4-7 d and VC received a P4 device for 7 days (Day 24 to 31). All females received intramuscularly 2.0 mg of estradiol benzoate (BE; Gonadiol, Schering Plough) on day 24 of the protocol. All devices were removed previously follicular aspiration (Day 31). At the time of OPU, ovaries were examined by ultrasonography to estimate follicular population. Data was analyzed with the procedure GLIMMIX of SAS and shown as means ± SEM. Means were assumed different when P<0.05. The number of recruited follicles (43.3±2.0 vs 58.9±2.4 vs 58.1±2.4 vs 58.2±2.7, P<0.001), number of total oocytes (25.9±1.5 vs 37.1±1.9 vs 41.4±2.3 vs 33.1±2.0, P<0.001), and number of viable oocytes (15.9±1.2 vs 26.1±1.6 vs 30.8±1.8 vs 22.5±1.7, P<0.001) was less in PP-P4-24d than in PP-P4-7d, PP, and VC, respectively. However, there were no differences among groups in terms of total embryos produced per OPU session (4.7±0.7 vs 4.4±0.7 vs 5.1±0.7 vs 4±0.7, respectively for PP-P4-24d, PP-P4-7d, PP, and VC, P=0.7). In addition, the embryo rate (number of embryos/number of viable oocytes) was lower (P<0,001) in PP-P4-7d (18.1%) and PP (15.3%) in comparison to PP-P4-24d (29.7%) and VC (25.9%) groups. As a conclusion, prepubertal Nelore heifers exposed to 24 days of progesterone previously to the OPU presented lower number of follicles and recovered oocytes but higher in vitro embryo production rates." @default.
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- W2733985235 date "2014-03-11" @default.
- W2733985235 modified "2023-09-27" @default.
- W2733985235 title "Effect of primer progesterone in oocyte quality and in vitro embryo production of Nelore cattle" @default.
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