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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2733992011> ?p ?o ?g. }

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• W2733992011 abstract "A described simple and advanced protocol for the substituted-cysteine accessibility method as applied to transmembrane (TM) orientation (SCAM™) permits a topology analysis of proteins in their native state and can be universally adapted to any membrane system to either systematically map an uniform topology or identify and quantify the degree of mixed topology. In this approach, noncritical individual amino acids that are thought to reside in the putative extracellular or intracellular loops of a membrane protein are replaced one at a time by cysteine residue, and the orientation with respect to the membrane is evaluated using a pair of membrane-impermeable nondetectable and detectable thiol-reactive labeling reagents." @default.
• W2733992011 created "2017-07-14" @default.
• W2733992011 creator A5035453308 @default.
• W2733992011 date "2017-01-01" @default.
• W2733992011 modified "2023-10-15" @default.
• W2733992011 title "Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM™)" @default.
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• W2733992011 doi "https://doi.org/10.1007/978-1-4939-7033-9_9" @default.
• W2733992011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28667607" @default.
• W2733992011 hasPublicationYear "2017" @default.
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