FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2733997966> ?p ?o ?g. }

• W2733997966 endingPage "1188" @default.
• W2733997966 startingPage "1169" @default.
• W2733997966 abstract "Treatment of leishmaniasis involves the use of antimonials, miltefosine, amphotericin B or pentamidine. However, the side effects of these drugs and the reports of drug-resistant parasites demonstrate the need for new treatments that are safer and more efficacious. Histone deacetylase inhibitors are a new class of compounds with potential to treat leishmaniasis. Herein, we evaluated the effects of KH-TFMDI, a novel histone deacetylase inhibitor, on Leishmania amazonensis promastigotes and intracellular amastigotes. The IC50 values of this compound for promastigotes and intracellular amastigotes were 1.976 and 1.148 μM, respectively, after 72 h of treatment. Microscopic analyses revealed that promastigotes became elongated and thinner in response to KH-TFMDI, indicating changes in cytoskeleton organization. Immunofluorescence microscopy, western blotting and flow cytometry using an anti-acetylated tubulin antibody revealed an increase in the expression of acetylated tubulin. Furthermore, transmission electron microscopy revealed several ultrastructural changes, such as (a) mitochondrial swelling, followed by the formation of many vesicles inside the matrix; (b) presence of lipid bodies randomly distributed through the cytoplasm; (c) abnormal chromatin condensation; and (d) formation of blebs on the plasma membrane. Physiological studies for mitochondrial function, flow cytometry with propidium iodide and TUNEL assay confirmed the alterations in the mitochondrial metabolism, cell cycle, and DNA fragmentation, respectively, which could result to cell death by mechanisms related to apoptosis-like. All these together indicate that histone deacetylases are promising targets for the development of new drugs to treat Leishmania, and KH-TFMDI is a promising drug candidate that should be tested in vivo." @default.
• W2733997966 created "2017-07-14" @default.
• W2733997966 creator A5007905696 @default.
• W2733997966 creator A5014123016 @default.
• W2733997966 creator A5021718033 @default.
• W2733997966 creator A5038009828 @default.
• W2733997966 creator A5046572292 @default.
• W2733997966 creator A5075280201 @default.
• W2733997966 creator A5090881457 @default.
• W2733997966 date "2017-07-06" @default.
• W2733997966 modified "2023-10-01" @default.
• W2733997966 title "KH-TFMDI, a novel sirtuin inhibitor, alters the cytoskeleton and mitochondrial metabolism promoting cell death in Leishmania amazonensis" @default.
• W2733997966 cites W1491563504 @default.
• W2733997966 cites W1850578074 @default.
• W2733997966 cites W1966377705 @default.
• W2733997966 cites W1971339883 @default.
• W2733997966 cites W1977068148 @default.
• W2733997966 cites W1982338402 @default.
• W2733997966 cites W1984963280 @default.
• W2733997966 cites W1989666486 @default.
• W2733997966 cites W1992708343 @default.
• W2733997966 cites W1993326216 @default.
• W2733997966 cites W1995176982 @default.
• W2733997966 cites W1996395636 @default.
• W2733997966 cites W1997959217 @default.
• W2733997966 cites W1999536542 @default.
• W2733997966 cites W2007226778 @default.
• W2733997966 cites W2007415161 @default.
• W2733997966 cites W2012233199 @default.
• W2733997966 cites W2013434401 @default.
• W2733997966 cites W2015010767 @default.
• W2733997966 cites W2015286563 @default.
• W2733997966 cites W2028514900 @default.
• W2733997966 cites W2032216634 @default.
• W2733997966 cites W2033241989 @default.
• W2733997966 cites W2036605603 @default.
• W2733997966 cites W2045694545 @default.
• W2733997966 cites W2062950876 @default.
• W2733997966 cites W2077766479 @default.
• W2733997966 cites W2081440055 @default.
• W2733997966 cites W2085578725 @default.
• W2733997966 cites W2110118637 @default.
• W2733997966 cites W2121274011 @default.
• W2733997966 cites W2122784293 @default.
• W2733997966 cites W2126799171 @default.
• W2733997966 cites W2165398964 @default.
• W2733997966 cites W2167441930 @default.
• W2733997966 cites W2168702014 @default.
• W2733997966 cites W2952410637 @default.
• W2733997966 cites W47495918 @default.
• W2733997966 doi "https://doi.org/10.1007/s10495-017-1397-8" @default.
• W2733997966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28685254" @default.
• W2733997966 hasPublicationYear "2017" @default.
• W2733997966 type Work @default.
• W2733997966 sameAs 2733997966 @default.
• W2733997966 citedByCount "23" @default.
• W2733997966 countsByYear W27339979662018 @default.
• W2733997966 countsByYear W27339979662019 @default.
• W2733997966 countsByYear W27339979662020 @default.
• W2733997966 countsByYear W27339979662021 @default.
• W2733997966 countsByYear W27339979662022 @default.
• W2733997966 countsByYear W27339979662023 @default.
• W2733997966 crossrefType "journal-article" @default.
• W2733997966 hasAuthorship W2733997966A5007905696 @default.
• W2733997966 hasAuthorship W2733997966A5014123016 @default.
• W2733997966 hasAuthorship W2733997966A5021718033 @default.
• W2733997966 hasAuthorship W2733997966A5038009828 @default.
• W2733997966 hasAuthorship W2733997966A5046572292 @default.
• W2733997966 hasAuthorship W2733997966A5075280201 @default.
• W2733997966 hasAuthorship W2733997966A5090881457 @default.
• W2733997966 hasBestOaLocation W27339979662 @default.
• W2733997966 hasConcept C104317684 @default.
• W2733997966 hasConcept C136764020 @default.
• W2733997966 hasConcept C153911025 @default.
• W2733997966 hasConcept C190283241 @default.
• W2733997966 hasConcept C203014093 @default.
• W2733997966 hasConcept C2775934118 @default.
• W2733997966 hasConcept C2776202225 @default.
• W2733997966 hasConcept C2776555147 @default.
• W2733997966 hasConcept C2778305200 @default.
• W2733997966 hasConcept C2778689377 @default.
• W2733997966 hasConcept C2781092759 @default.
• W2733997966 hasConcept C2781417161 @default.
• W2733997966 hasConcept C31573885 @default.
• W2733997966 hasConcept C41008148 @default.
• W2733997966 hasConcept C55493867 @default.
• W2733997966 hasConcept C64927066 @default.
• W2733997966 hasConcept C71928629 @default.
• W2733997966 hasConcept C79879829 @default.
• W2733997966 hasConcept C86803240 @default.
• W2733997966 hasConcept C95444343 @default.
• W2733997966 hasConcept C98319499 @default.
• W2733997966 hasConceptScore W2733997966C104317684 @default.
• W2733997966 hasConceptScore W2733997966C136764020 @default.
• W2733997966 hasConceptScore W2733997966C153911025 @default.
• W2733997966 hasConceptScore W2733997966C190283241 @default.
• W2733997966 hasConceptScore W2733997966C203014093 @default.
• W2733997966 hasConceptScore W2733997966C2775934118 @default.

• next