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• W2734213403 endingPage "13242" @default.
• W2734213403 startingPage "13230" @default.
• W2734213403 abstract "The PAH1-encoded phosphatidate phosphatase (PAP), which catalyzes the committed step for the synthesis of triacylglycerol in Saccharomyces cerevisiae, exerts a negative regulatory effect on the level of phosphatidate used for the de novo synthesis of membrane phospholipids. This raises the question whether PAP thereby affects the expression and activity of enzymes involved in phospholipid synthesis. Here, we examined the PAP-mediated regulation of CHO1-encoded phosphatidylserine synthase (PSS), which catalyzes the committed step for the synthesis of major phospholipids via the CDP–diacylglycerol pathway. The lack of PAP in the pah1Δ mutant highly elevated PSS activity, exhibiting a growth-dependent up-regulation from the exponential to the stationary phase of growth. Immunoblot analysis showed that the elevation of PSS activity results from an increase in the level of the enzyme encoded by CHO1. Truncation analysis and site-directed mutagenesis of the CHO1 promoter indicated that Cho1 expression in the pah1Δ mutant is induced through the inositol-sensitive upstream activation sequence (UASINO), a cis-acting element for the phosphatidate-controlled Henry (Ino2–Ino4/Opi1) regulatory circuit. The abrogation of Cho1 induction and PSS activity by a CHO1 UASINO mutation suppressed pah1Δ effects on lipid synthesis, nuclear/endoplasmic reticulum membrane morphology, and lipid droplet formation, but not on growth at elevated temperature. Loss of the DGK1-encoded diacylglycerol kinase, which converts diacylglycerol to phosphatidate, partially suppressed the pah1Δ-mediated induction of Cho1 and PSS activity. Collectively, these data showed that PAP activity controls the expression of PSS for membrane phospholipid synthesis." @default.
• W2734213403 created "2017-07-14" @default.
• W2734213403 creator A5015880535 @default.
• W2734213403 creator A5047376925 @default.
• W2734213403 date "2017-08-01" @default.
• W2734213403 modified "2023-10-12" @default.
• W2734213403 title "Yeast PAH1-encoded phosphatidate phosphatase controls the expression of CHO1-encoded phosphatidylserine synthase for membrane phospholipid synthesis" @default.
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• W2734213403 doi "https://doi.org/10.1074/jbc.m117.801720" @default.
• W2734213403 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5555185" @default.
• W2734213403 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28673963" @default.
• W2734213403 hasPublicationYear "2017" @default.
• W2734213403 type Work @default.

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