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- W2734459201 abstract "Summary It is well documented that both epidermal growth factor (EGF) and glial cell line-derived neurotrophic factor (GDNF) are critical for porcine oocyte maturation, however, little information is known about their mechanism of action in vitro . To gain insight into the mechanisms of action of the optimum doses of EGF and GDNF on porcine oocyte maturation, porcine cumulus–oocyte complexes (COCs) were matured in defined porcine oocyte medium supplemented with EGF, GDNF or a combination of both at varying concentrations (0–100 ng/ml) for 44 h. Nuclear and cytoplasmic maturation were determined in terms of nuclear stage after DNA staining with Hoechst and cortical granule distribution after lectin labeling, respectively. Mature oocytes were subsequently collected for gene expression analysis or subjected to in vitro fertilization and cultured for 7 days. The results showed that EGF and/or GDNF, when administered in a certain dose (50 ng/μl) to the maturation medium, not only effectively improved the synchronization of nuclear and cytoplasmic maturation processes within the oocyte, but enhanced expression of their corresponding receptors in mature oocytes ( P < 0.05). Moreover, supplementation with an optimal combination of EGF + GDNF resulted in elevation of TFAM transcripts as well as a decrease of caspase-3 transcripts compared with the other studied groups ( P < 0.05). Collectively, our results indicate that treatment of porcine oocytes with specific-dose combinations of EGF and GDNF stimulates oocyte quality and competence by transcriptional modulation of genes involved in oocyte survival and competence." @default.
- W2734459201 created "2017-07-21" @default.
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- W2734459201 date "2017-07-11" @default.
- W2734459201 modified "2023-09-26" @default.
- W2734459201 title "Optimal doses of EGF and GDNF act as biological response modifiers to improve porcine oocyte maturation and quality" @default.
- W2734459201 cites W1892324454 @default.
- W2734459201 cites W1973696025 @default.
- W2734459201 cites W1979628427 @default.
- W2734459201 cites W1980914859 @default.
- W2734459201 cites W1989381407 @default.
- W2734459201 cites W2000105010 @default.
- W2734459201 cites W2001849758 @default.
- W2734459201 cites W2003252770 @default.
- W2734459201 cites W2005868479 @default.
- W2734459201 cites W2006357476 @default.
- W2734459201 cites W2019866143 @default.
- W2734459201 cites W2020552473 @default.
- W2734459201 cites W2024560367 @default.
- W2734459201 cites W2026774999 @default.
- W2734459201 cites W2030460116 @default.
- W2734459201 cites W2035510998 @default.
- W2734459201 cites W2038589125 @default.
- W2734459201 cites W2049166996 @default.
- W2734459201 cites W2049832142 @default.
- W2734459201 cites W2053174155 @default.
- W2734459201 cites W2054196079 @default.
- W2734459201 cites W2055073243 @default.
- W2734459201 cites W2056555106 @default.
- W2734459201 cites W2061167268 @default.
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- W2734459201 cites W2071484008 @default.
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- W2734459201 cites W2074140943 @default.
- W2734459201 cites W2074261055 @default.
- W2734459201 cites W2079331220 @default.
- W2734459201 cites W2082649278 @default.
- W2734459201 cites W2083205981 @default.
- W2734459201 cites W2088235456 @default.
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- W2734459201 cites W2158723358 @default.
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- W2734459201 cites W2169430457 @default.
- W2734459201 cites W2169733234 @default.
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- W2734459201 doi "https://doi.org/10.1017/s0967199417000181" @default.
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